HILIC-MS-MS测定不同产地酒制多花黄精中游离氨基酸成分含量  

Content Determination of Free Amino Acids in Polygonatum cyrtonema from Different Regions by Using HILIC-MS/MS

作　　者：刘嘉钰 李思梦 罗雨欣 陈光宇[1] 谢梦洲[1] LIU Jia-yu;LI Si-meng;LUO Yu-xin;CHEN Guang-yu;XIE Meng-zhou(Hunan University of Chinese Medicine/Engineering Technology Research Center For Medicinal and Functional Food,Hunan University of Chinese Medicine/Key Laboratory of TCM Heart and Lung Syndrome Differentiation&Medicated Diet and Dietotherapy,Hunan University of Chinese Medicine/Provincial Key Laboratory of TCM Diagnostics,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学/湖南中医药大学药食同源功能性食品工程技术研究中心/湖南中医药大学中医心肺病证辨证与药膳食疗重点研究室/湖南中医药大学中医诊断学湖南省重点实验室,长沙410208

出　　处：《中国食物与营养》2025年第3期50-55,共6页Food and Nutrition in China

基　　金：湖南省重点研发计划(2021-2022年)项目(第二批)(项目编号:2022SK2018);2023年度湖南省中医药科研课题(项目编号:B2023134)。

摘　　要：目的:建立不同产地酒制多花黄精中12种游离氨基酸成分的含量测定方法。方法:采用亲水色谱-三重四极杆质谱法(HILIC-MS/MS)测定新化(野生)、南平、郴州、邵阳、湘西、凯里、新化(人工)、岳阳、眉山、玉溪、铜仁等11个产地酒制多花黄精中L-丙氨酸、L-精氨酸、L-谷氨酸、L-甘氨酸、L-组氨酸、L-赖氨酸、L-苯丙氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸、L-酪氨酸、L-缬氨酸等12种游离成分含量,采用多组独立样本比较的秩和检验评价不同产地酒制多花黄精12种成分含量高低,主成分(PCA)分析差异性。结果:所建的含量测定方法良好,12种游离氨基酸含量之和在0.6~4.8 mg/g之间,L-苏氨酸、L-精氨酸含量相对较高,11个产酒制多花黄精中12种成分由高到低排序为:新野>邵阳>玉溪>新林>铜仁>南平>湘西>凯里>眉山>郴州>岳阳,L-丙氨酸和L-组氨酸为对PCA差异性贡献度最大的两个成分。结论:建立了酒制多花黄精中12种游离氨基酸成分的含量测定方法,比较了不同产地酒制多花黄精中氨基酸成分的差异,可为多花黄精质量标准研究、资源分布及产品开发提供实验依据。【Objective】To establish a method for the content determination of 12 free amino acids in the Polygonatum cyrtonema from different producing areas.【Method】Totally 12 free amino acids of L-alanine,L-arginine,L-glutamic acid,L-glycine,L-histidine,Llysine,L-phenylalanine,L-proline,L-serine,L-threonine,L-tyrosine,L-valine were determined by Hydrophilic Chromatography-Triple Quadrupole Mass Spectrometry(HILIC-MS/MS)in the Polygonatum cyrtonema from 11 kinds of producing areas from XY,NP,CZ,SY,XX,KL,XL,YY,MS,YX,TR.The content of 12 components was evaluated by the Rank Sum Test of Multiple Independent Samples in the Polygonatum cyrtonema from different producing areas,and the difference was analysed by Principal Component Analysis(PCA).【Result】The established method of content determination was good.The content of 12 kinds of free amino acids in Polygonatum cyrtonema ranged from 0.6~4.8 mg/g.The content of L-threonine and L-arginine was relatively high.The 12 ingredients in Polygonatum cyrtonema were listed in descending order as XY>SY>YX>XL>TR>NP>XX>KL>MS>CZ>YY,and L-alanine and L-histidine were the most contributed to the difference in PCA.【Conclusion】The method for the determination of 12 free amino acids in the Polygonatum cyrtonema was established,and the differences of amino acids in Polygonatum cyrtonema from different producing areas were compared,which can provide experimental basis for quality standard research,resource distribution and product development of Polygonatum cyrtonema.

关 键 词：多花黄精 亲水作用色谱-串联质谱法 氨基酸 秩和检验 

分 类 号：R28[医药卫生—中药学]