基于线虫模型的流苏石斛和鼓槌石斛神经保护作用研究  

Study on The Protection of Dendrobium chrysotoxum and Dendrobium fimbriatum Against Neurodegenerative Diseases Based on Nematode Models

作　　者：刘鹏飞 陈程 王凤忠[1] 刘佳妮 杨洋[1] 王琼 何汶璐 孙晶 邹翔[2] 范蓓[1] LIU Peng-fei;CHEN Cheng;WANG Feng-zhong;LIU Jia-ni;YANG Yang;WANG Qiong;HE Wen-lu;SUN Jing;ZOU Xiang;FAN Bei(Institute of Food Science and Technology,CAAS/Key Laboratory of Agro-Products Quality and Safety in Harvest,Storage,Transportation,Management and Control,Ministry of Agriculture and Rural Affairs,Beijing 100193,China;Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin University of Commerce,Harbin 150000,China;Southwest Medical University Sino-Portuguese Chinese Medicine International Cooperation Center,Luzhou 646000,China)

机构地区：[1]中国农业科学院农产品加工研究所/农业农村部农产品质量安全收贮运管控重点实验室,北京100193 [2]哈尔滨商业大学国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150000 [3]西南医科大学中葡中医药国际合作中心,四川泸州646000

出　　处：《中国食物与营养》2025年第3期76-81,共6页Food and Nutrition in China

基　　金：国家重点研发计划(项目编号:2022YFD1600303、2022YFC2010104);国家自然科学基金(项目编号:32101957);中国农业科学院农产品加工研究所创新工程院所重点任务(项目编号:CAAS-ASTIP-G2022-IFST-04);黑龙江省重点研发计划指导类项目(项目编号:GZ20210110)。

摘　　要：目的:探讨不同浓度的鼓槌石斛乙醇提取物(DCLA)、鼓槌石斛水提物(DCLW)、流苏石斛乙醇提取物(DFHA)、流苏石斛水提物(DFHW)对秀丽隐杆线虫α-突触核蛋白聚集的抑制作用。方法:以秀丽隐杆线虫为模型生物,实验分为空白组、左旋多巴阳性药组和石斛提取物组,检测0.1、1、10、100 mg/mL的DCLA、DCLW、DFHA、DFHW的黄色荧光蛋白荧光强度,检测其抗α-突触核蛋白聚集作用。结果:与空白组相比,DCLA、DCLW、DFHA、DFHW均对α-突触核蛋白聚集显示出显著抑制作用,且在各实验组中,10 mg/mL均为最佳抑制浓度,其抑制活性由大到小为DCLA>DFHA>DCLW>DFHW。从不同溶剂提取方式来看,乙醇提取物比水提物显示出更明显的抑制活性,可能是生物碱类和黄酮类成分发挥的作用。结论:鼓槌石斛和流苏石斛水提物及醇提物均有一定的神经保护作用,10 mg/mL DCLA抗α-突触核蛋白聚集作用最强,石斛中的生物碱类和黄酮类可能是神经保护的活性成分。【Objective】To study the inhibitory effects of ethanol extract of Dendrobium chrysotoxum(DCLA),water extract of Dendrobium chrysotoxum(DCLW),ethanol extract of Dendrobium fimbriatum(DFHA),and water extract of Dendrobium fimbriatum(DFHW)on Caenorhabditis elegans’α-synapse aggregation in different concentrations.【Method】Using C.elegans as model organism,the experiment was divided into blank group,levodopa positive drug group and Dendrobium extract group.The fluorescence intensity of yellow fluorescent protein of DCLA,DCLW,DFHA and DFHW of 0.1 mg/mL,1 mg/mL,10 mg/mL and 100 mg/mL were detected.The anti-α-synaptonucleoprotein aggregation was detected.【Result】Compared with the blank group,DCLA,DCLW,DFHA,and DFHW showed significant inhibitory effects onα-synuclein aggregation,and in all experimental groups,10 mg/mL was the best inhibitory concentration,and their inhibitory activities were DCLA>DFHA>DCLW>DFHW in descending order.From the different solvent extracts,the ethanol extract showed more obvious inhibitory activity than water extract,which may be the role of alkaloids and flavonoids.【Conclusion】Both the aqueous and alcoholic extracts of Dendrobium drumstick and Dendrobium frutescens showed some neuroprotective effects,and 10 mg/mL DCLA had the strongest effect againstα-synuclein aggregation.The alkaloids and flavonoids in DCLA may be the active components of neuroprotective effects.

关 键 词：鼓槌石斛 流苏石斛 Α-突触核蛋白 神经保护 

分 类 号：R28[医药卫生—中药学]