应用大蒜素缓解牛子宫内膜炎损伤的研究  

Study on Alleviating Bovine Endometritis Injury with Allicin

作　　者：楚秋霞[1] 陈付英[1] 冯亚杰[1] 张子敬[1] 师志海[1] 施巧婷[1] 王二耀[1] 吕世杰 付晓鹤 CHU Qiu-xia;CHEN Fu-ying;FENG Ya-jie(Institute of Animal Husbandry,Henan Academy of Agricultural Sciences/Henan Provincial Key Laboratory of Livestock and Poultry Breeding and Nutrition Regulation,Zhengzhou,Henan 450002)

机构地区：[1]河南省农业科学院畜牧研究所/河南省畜禽繁育与营养调控重点实验室,河南郑州450002 [2]郑州市兽药饲料质量安全检验中心,河南郑州450000

出　　处：《安徽农业科学》2025年第6期85-88,共4页Journal of Anhui Agricultural Sciences

基　　金：国家肉牛牦牛产业技术体系项目(CARS-37);河南省肉牛产业技术体系项目(HARS-22-13-S);河南省农科院自主创新项目(2024ZC090)。

摘　　要：[目的]研究大蒜素对牛子宫内膜炎损伤的治疗作用。[方法]采用脂多糖诱导牛子宫内膜上皮细胞建立炎性细胞模型,探讨大蒜素的抗炎机制。筛选最佳LPS浓度;通过ELISA方法、荧光定量PCR技术和Western blot方法分别检测细胞炎症因子的含量、mRNA相对表达量和蛋白相对表达量。[结果]不同浓度的LPS均能够提高细胞的增殖量,当LPS浓度为30μg/mL时细胞活力最高。与炎性细胞相比,大蒜素可抑制LPS诱导的子宫内膜上皮细胞中TNF-α、IL-1β、IL-6和IL-8炎性因子的分泌,也能降低IL-1β、TLR4和NF-κβp65的基因转录水平。Western blot分析结果表明,大蒜素能够抑制TLR4和NF-κβp65蛋白的表达。[结论]大蒜素能够抑制TLR4/NF-κβ信号通路,通过调节细胞炎症因子的分泌发挥抗炎活性。[Objective]To study the treatment effect of allicin on bovine endometritis injury.[Method]Bovine endometrial epithelial cells were induced by lipopolysaccharide to establish an inflammatory cell model,and explore the anti-inflammatory mechanism of allicin.The optimal concentration of LPS was screened out.The secretion,mRNA relative expression and protein relative expression of inflammatory factors were detected by ELISA,fluorescence quantitative PCR and Western blot method.[Result]Different concentrations of LPS could improve cell proliferation,and the cell viability was the highest when LPS concentration was 30μg/mL.Compared with inflammatory cells,allicin could inhibit the secretion of inflammatory factors TNF-α,IL-1β,IL-6 and IL-8 in LPS-induced endometrial epithelial cells,and also reduced the gene transcription levels of IL-1β,TLR4,and NF-κβp65.Allicin inhibited the expression of TLR4 and NF-κβp65 proteins by Western blot analysis.[Conclusion]Allicin can inhibit the TLR4/NF-κβsignaling pathway and exert anti-inflammatory activity by regulating the secretion of inflammatory cytokines in cells.

关 键 词：牛子宫内膜上皮细胞 大蒜素 炎性反应 细胞因子 

分 类 号：S858.23[农业科学—临床兽医学]