1株犬冠状病毒山西株变异与遗传进化特征  

Gene variation and genetic evolution characteristics of a canine coronavirus strain in Shanxi Province

作　　者：刘晶英 李炜 杨勃 孙子龙[2] 张鼎[2] LIU Jingying;LI Wei;YANG Bo;SUN Zilong;ZHANG Ding(School of Basic Medicine,Shanxi Medical University,Taiyuan 030001,China;College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西农业大学动物医学学院,山西太谷030801

出　　处：《畜牧与兽医》2025年第4期85-92,共8页Animal Husbandry & Veterinary Medicine

基　　金：山西省回国留学人员科研资助项目(2023-094)。

摘　　要：旨在阐明分离自山西省的1株犬冠状病毒(CCoV)基因变异与遗传进化特征。将疑似CCoV感染犬粪便接种犬肾传代细胞系(MDCK),收毒后扩增CCoV山西株纤突蛋白(S)基因、膜蛋白(M)基因,分别利用DNASTAR、ESPript 3.x和MEGA-X软件分析基因、氨基酸相似性以及遗传进化关系。结果:CCoV山西株S基因与已公布参考序列的相似性为88.6%~98.3%,M基因相似性为83.6%~96.3%;CCoV山西株S蛋白、M蛋白均存在部分氨基酸位点变异,其中S蛋白主要为氨基酸点突变,M蛋白第10~40位点呈现高频氨基酸突变区;邻近法(Neighbor-Joining)构建遗传进化树结果显示,CCoV山西株属于CCoVⅡ型毒株,与越南分离株LC190907处于同一分支。综上,S基因、M基因分析结果一致,均表明该CCoV山西株属于CCoVⅡ型毒株,存在不同程度的核苷酸、氨基酸变异,与越南分离株亲缘关系最近。本研究结果为山西省CCoV相关疾病的防控提供了重要的参考。To elucidate the homology and genetic variation of a canine coronavirus(CCoV)strain in Shanxi Province,the present study amplified the S gene and the M gene of the CCoV Shanxi strain,analyzing the gene,amino acid homology and genetic evolution of the CCoV Shanxi strain using the DNASTAR,ESPript 3.x and MEGA-X softwares,respectively.The results showed that the S gene homology of the CCoV Shanxi strain with published reference sequences was 88.6%-98.3%,and its M gene homology was 83.6%-96.3%.The S protein and M protein of the CCoV Shanxi strain had partial amino acid site variation,among which the S protein was mainly amino acid point muta⁃tion,and the M protein showed a continuous amino acid mutation region at sites of 10 to 40.The genetic evolutionary tree constructed using the Neighbor-Joining method showed that the CCoV Shanxi strain belonged to the CCoV typeⅡstrain,which was in the same branch with that of the CCoV isolated in Vietnam(LC190907).In summary,The analysis results of the S gene and M gene were consistent,which indi⁃cated that the CCoV Shanxi strain belonged to CCoV typeⅡstrain,with different degrees of gene and amino acid variation,and was closely related to the strain isolated in Vietnam.The findings of the present study provided an important theoretical reference for the study of CCoV etiology and disease control in Shanxi Province.

关 键 词：犬冠状病毒 纤突蛋白基因 膜蛋白基因 同源性 遗传进化 

分 类 号：S852.65[农业科学—基础兽医学]