鹅掌楸苷对缺血再灌注大鼠心功能的保护作用与机制  

Protective effects and mechanisms of liriodendrin on cardiac function in rats with ischemia/reperfusion injury

作　　者：姚博宸 秦名震 马晓中 陈庆良[1] 李博[1] 郭志刚[1] YAO Bochen;QIN Mingzhen;MA Xiaozhong;CHEN Qingliang;LI Bo;GUO Zhigang(Department of Cardiac Surgery,Tianjin Chest Hospital,Tianjin 300001,China)

机构地区：[1]天津市胸科医院心脏外科,天津300001

出　　处：《临床与病理杂志》2024年第11期1506-1515,共10页Journal of Clinical and Pathological Research

摘　　要：目的:有关鹅掌楸苷对于缺血再灌注(ischemia/reperfusion,I/R)后心肌细胞是否具有保护作用及其作用机制的研究较少。本研究拟探讨鹅掌楸苷对I/R大鼠心功能的保护作用及其可能的机制。方法:将大鼠随机分为假手术组、I/R组、鹅掌楸苷+I/R组和丹参滴丸+I/R组。鹅掌楸苷+I/R组及丹参滴丸+I/R组于术前5 d至术后3 d,每日予以鹅掌楸苷溶液灌胃1次,I/R组及假手术组采用每日10 mL/kg生理盐水灌胃。术后分别对假手术组、I/R组和鹅掌楸苷+I/R组动物进行超敏肌钙蛋白定量检测。术后3 d应用超声心动图对大鼠心功能进行检测。处死大鼠后行苏木精-伊红(hematoxylin-eosin,HE)染色和免疫组织化学(immunohistochemistry,IHC)染色。进一步应用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)对组织行白介素(interleukin,IL)-1β、肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)、IL-6、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)、活性氧(reactive oxygen species,ROS)和乳酸脱氢酶A(lactate dehydrogenase A,LDHA)进行检测。应用蛋白质印迹法检测组织的炎症通路及凋亡相关蛋白的表达。4组大鼠均采用real-time PCR检测相关基因分子的相对mRNA表达。结果:与I/R组比较,术后2 h鹅掌楸苷+I/R组肌钙蛋白显著降低(P<0.05)。心脏超声显示:与I/R组比较,鹅掌楸苷+I/R组的心功能显著好转,病理学染色显示I/R组的心肌组织破坏严重,大量炎症细胞浸润。ELISA结果显示:与I/R组比较,鹅掌楸苷+I/R组的IL-1β、TNF-α、IL-6、MCP-1、ROS和LDHA含量均降低(均P<0.05)。与I/R组比较,鹅掌楸苷+I/R组炎症及凋亡相关蛋白表达均降低(均P<0.05)。与假手术组比较,I/R组、鹅掌楸苷+I/R组和丹参滴丸+I/R组B-淋巴细胞瘤-2基因(B-cell lymphoma-2,BCL-2)的mRNA相对表达量显著降低(均P<0.05);与I/R组比较,鹅掌楸苷+I/R组BCL-2 mRNA相对表达量显著升高(P<0.05)。与假手术组比�Objective:Research on whether liriodendrin exerts a protective effect on myocardial cells following ischemia/reperfusion(I/R)injury and its underlying mechanisms is limited.This study aims to explore the protective effects and potential mechanisms of liriodendrin on cardiac function in rats subjected to I/R injury.Methods:Rats were randomly divided into 4 groups:A sham surgery group,a I/R group,a liriodendrin+I/R group,and a Danshen dripping pill+I/R group.The liriodendrin+I/R and Danshen dripping pill+I/R groups received liriodendrin solution by gavage once daily from 5 days before surgery to 3 days after surgery,whereas the I/R group and the sham group received 10 mL/kg saline daily by gavage.Postoperatively,high-sensitivity cardiac troponin levels were quantified in the sham surgery group,the I/R group,and the liriodendrin+I/R group,and echocardiography was performed on day 3 to assess cardiac function.After euthanasia,hematoxylin-eosin(HE)staining and immunohistochemistry(IHC)staining were performed.Enzyme-linked immunosorbent assay(ELISA)was used to measure interleukin(IL)-1 beta(IL-1β),tumor necrosis factor-alpha(TNF-α),IL-6,monocyte chemoattractant protein-1(MCP-1),reactive oxygen species(ROS),and lactate dehydrogenase A(LDHA)levels in cardiac tissues.Western blotting was used to evaluate the expression of inflammation and apoptosis-related proteins,while real-time PCR was employed to assess relative mRNA expression levels of target genes across all four groups.Results:Compared with the I/R group,cardiac troponin levels in the liriodendrin+I/R group were significantly reduced 2 hours postoperatively(P<0.05).Echocardiography showed significant cardiac function improvement in the liriodendrin+I/R group.Pathological staining revealed severe myocardial damage and extensive inflammatory cell infiltration in the I/R group.ELISA results showed that IL-1β,TNF-α,IL-6,MCP-1,ROS,and LDHA levels were significantly lower in the liriodendrin+I/R group compared to the I/R group(all P<0.05).Additionally,inflammation a

关 键 词：冠心病 缺血再灌注 鹅掌楸苷 细胞凋亡 炎症因子 炎症通路 

分 类 号：R73[医药卫生—肿瘤]