A PRA-Rab trafficking machinery modulates NLR immune receptor plasma membrane microdomain anchoring and blast resistance in rice  

PRA-Rab介导的囊泡转运途径调控NLR免疫受体的微区定位和水稻对稻瘟病的抗性

作　　者：Di Liang Dongyong Yang Tai Li Zhe Zhu Bingxiao Yan Yang He Xiaoyuan Li Keran Zhai Jiyun Liu Yoji Kawano Yiwen Deng Xu Na Wu Junzhong Liu Zuhua He 梁迪;杨冬勇;李泰;朱喆;颜丙;何洋;李晓媛;翟科然;刘继云;Yoji Kawano;邓一文;武旭娜;刘军钟;何祖华

机构地区：[1]CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China [2]University of the Chinese Academy of Sciences,Beijing 100049,China [3]Yunnan Key Laboratory of Cell Metabolism and Diseases,State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan,Center for Life Sciences,School of Life Sciences,Yunnan University,Kunming 650500,China [4]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China [5]Institute of Plant Science and Resources,Okayama University,Okayama 710-0046,Japan [6]College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China

出　　处：《Science Bulletin》2025年第5期733-747,共15页科学通报(英文版)

基　　金：Biological Breeding-National Science and Technology Major Projects(2023ZD04070);Chinese Academy of Sciences(XDA24010304 and XDB27040201);National Natural Science Foundation of China(32088102,31930090,31720103913,and 32070564);National Key Research and Development Program of China(2016YFD0100600);Postdoctoral Innovation Talents Support Program(BX20240376);Xingdian Talent Support Program of Yunnan Province(C619300A094);Yunnan Fundamental Research Projects(202301BF070001-013,202101AW070002,202201AT070090 and 202301AT070166).

摘　　要：Nucleotide-binding leucine-rich repeat(NLR)receptors mediate pathogen effector-triggered immunity(ETI)in plants,and a subclass of NLRs are hypothesized to function at the plasma membrane(PM).However,how NLR traffic and PM delivery are regulated during immune responses remains largely unknown.The rice NLR PigmR confers broad-spectrum resistance to the blast fungus Magnaporthe oryzae.Here,we report that a PRA(Prenylated Rab acceptor)protein,PIBP4(PigmR-INTERACTING and BLAST RESISTANCE PROTEIN 4),interacts with both PigmR and the active form of the Rab GTPase,OsRab5a,thereby loads a portion of PigmR on trafficking vesicles that target to PM microdomains.Microdomain-localized PigmR interacts with and activates the small GTPase OsRac1,which triggers reactive oxygen species signaling and hypersensitive response,leading to immune responses against blast infection.Thus,our study discovers a previously unknown mechanism that deploys a PRA-Rab protein delivering hub to ensure ETI,linking the membrane trafficking machinery with NLR function and immune activation in plants.

关 键 词：Prenylated Rab acceptor PigmR Trafficking vesicles OsRab5a Blast resistance 

分 类 号：R73[医药卫生—肿瘤]