2023年江苏省一起登革热疫情的病毒分子特征分析  

Analysis of the viral molecular characteristics in a dengue fever outbreak in Jiangsu province in 2023

作　　者：秦圆方[1] 张楠 甄茜[2] 李志锋[1] 居豪 朱立国[1] 付建光[1] Qin Yuanfang;Zhang Nan;Zhen Qian;Li Zhifeng;Ju Hao;Zhu Liguo;Fu Jianguang(Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009;Changzhou Center for Disease Control and Prevention,Changzhou 213022,China)

机构地区：[1]江苏省疾病预防控制中心,南京210009 [2]常州市疾病预防控制中心,常州213022

出　　处：《中华实验和临床病毒学杂志》2025年第1期81-85,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的对2023年江苏省出现的一起登革热本地疫情的病毒分子特征,为疫情的防控提供依据。方法采集疑似登革热患者急性期的血清样本,通过实时荧光定量RT-PCR(RT-qPCR)进行病毒检测和血清分型。阳性标本扩增全长基因组片段,并进行二代测序和相关进化分析。结果江苏省常州市在2023年10月18日至21日发现4例登革确诊病例,病例间存在流行病学关联,认定为一起登革热疫情。4例患者血清RT-qPCR结果均为登革热1型,其中3例获得了全基因组序列。E基因与全基因组进化树显示3个序列均位于基因型1-Ⅰ型的第3分支上,与2023年广东、云南两省的登革病毒流行株高度同源。氨基酸变异位点分析显示这3个病例序列共有16个分支特有的氨基酸位点的改变,其中C蛋白和prM蛋白各有1个变异位点,E蛋白有2个,非结构蛋白中NS5变异位点最多(9个)。结论此次江苏本地疫情是由登革热1型病毒引起,核苷酸序列与国内其他地区的毒株相似性较高,同时出现了氨基酸位点的改变。ObjectiveTo analyze the molecular characteristics of the virus in a local outbreak of dengue fever in Jiangsu province in 2023,and to provide a basis for the prevention and control of the outbreak.MethodsSerum samples were collected from suspected dengue patients in the acute phase of the outbreak for virus detection and serotyping by real-time fluorescence quantitative RT-PCR(RT-qPCR).Positive specimens were amplified with full-length genomic fragments and subjected to second-generation sequencing and related evolutionary analyses.ResultsFour confirmed cases of dengue were found in Changzhou city,Jiangsu province,from October 18 to 21,2023,with epidemiological association between the cases,which was recognized as a dengue outbreak.The serum RT-qPCR result of the four cases were all dengue type 1,and the whole genome sequences of three of the cases were obtained.The evolutionary tree of the E gene and the whole genome showed that the three sequences were located in the 3rd branch of the 1-I genotype,which is similar to the genotype 1-I.The genome-wide sequences of the E gene and the genome-wide evolution tree showed that the three sequences were located in the 3rd branch of the 1-I genotype,which is similar to the genome-wide genotype 1-Ⅰ.The E gene and the genome-wide evolutionary tree showed that all three sequences were located on branch 3 of genotype 1-Ⅰ,with high sequence similarity to the dengue virus epidemic strains in Guangdong and Yunnan provinces in 2023.Amino acid variant site analysis showed that there were 16 branch-specific amino acid site changes in the sequences of the three cases,among which the structural proteins,C protein and prM protein,had one variant site each,E protein had two,and the non-structural proteins had the largest number of NS5 variant sites(9).ConclusionsThe local outbreak in Jiangsu was caused by dengue fever type 1 virus,with high nucleotide sequence similarity to strains from other regions of China,and amino acid site alterations.

关 键 词：登革热1型病毒 全基因组测序 基因分型 分子特征 

分 类 号：R51[医药卫生—内科学]