重组毕赤酵母表达杂合抗菌肽HsSP-TtT  

作　　者：上官雪茹 蔡依婕 李婉祺 张海乾 陈伟峰 曾臻 谭强来 

机构地区：[1]厦门医学院,福建厦门361023

出　　处：《科技创新与应用》2025年第9期60-63,68,共5页Technology Innovation and Application

基　　金：福建省自然科学基金青创项目(2022J05322);福建省中青年教师教育科研项目(JAT210492);厦门医学院大学生创新创业训练计划项目(202212631005,202212631006,202412631031)。

摘　　要：构建重组毕赤酵母pPICZαA-HsSP-TtT1/X33,表达杂合抗菌肽HsSP-TtT1。基于生物信息学分析和毕赤酵母密码子偏好性,设计杂合抗菌肽HsSP-TtT1的核酸序列,构建重组质粒pPICZαA-HsSP-TtT1,转化毕赤酵母X33,利用博来霉素抗性YPD平板筛选阳性转化子,经甲醇诱导后,SDS-PAGE凝胶电泳验证。成功构建重组质粒pPICZαA-HsSP-TtT1,并成功筛选出1株能够有效表达目标蛋白的重组毕赤酵母X33/pPICZαA-HsSP-TtT1菌株。该研究可为杂合抗菌肽HsSP-TtT1的高效制备提供物质基础。To construct a recombinant Pichia pastoris pPICZαA-HsSP Tt T1/X33 and express the hybrid antibacterial peptide HsSP Tt T1.Based on bioinformatics analysis and the codon preference of Pichia pastoris,the nucleic acid sequence of the hybrid antimicrobial peptide HsSP Tt T1 was designed,the recombinant plasmid pPICZ α A-HsSP Tt T1 was constructed,and Pichia pastoris X33 was transformed.The positive transformants were screened using the bleomycin resistant YPD plate.After methanol induction,they were verified by SDS-PAGE gel electrophoresis.The recombinant plasmid pPICZαA-HsSP-TtT1 were successfully constructed,and a recombinant strain of Pichia pastoris X33/pPICZαA-HsSP-Tt T1 which could effectively express the target protein was successfully screened.This study provides a material basis for the efficient preparation of the hybrid antimicrobial peptide HsSP-TtT1.

关 键 词：杂合抗菌肽 毕赤酵母 重组表达 HsSP TtT1 

分 类 号：Q816[生物学—生物工程]