基于响应面法优化益生菌发酵六神曲制备工艺  

To Optimize the Preparation Process of Massa Medicata Fermentata by Probiotic Fermentation Based on Response Surface Method

作　　者：吴金鹏 吴影[1,2,3] 张杰 樊秋霞 孙建瑞 田萍萍[1,2] 铁珊珊 古绍彬 WU Jinpeng;WU Ying;ZHANG Jie;FAN Qiuxia;SUN Jianrui;TIAN Pingping;TIE Shanshan;GU Shaobin(School of Food and Bioengineering,He'nan University of Science and Technology,Luoyang,He'nan 471000,China;He'nan Food Microbiology Engineering Technology Research Center,Luoyang,He'nan 471023,China;National Experimental Teaching Demonstration Center for Food Processing and Safety,Luoyang,He'nan 471023,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471000 [2]河南省食品微生物工程技术研究中心,河南洛阳471023 [3]食品加工与安全国家级实验教学示范中心,河南洛阳471023

出　　处：《农产品加工》2025年第5期31-36,43,共7页Farm Products Processing

基　　金：河南省自然科学基金项目(202300420429)。

摘　　要：采用3株产β-葡萄糖苷酶活力高的乳酸菌对六神曲进行强化发酵,利用正交试验确定其配比,通过单因素试验探究发酵时间、料液比、接种量、发酵温度、曲块体积对强化六神曲的影响。以淀粉酶活力为检测指标,通过Plackett-burman试验筛选影响六神曲制备工艺的关键因素,利用Box-behnken设计响应面法优化添加益生菌的六神曲制备工艺。结果表明,在发酵时间4 d,发酵温度32℃,料液比1∶1.1,接种量为3%条件下所制备的益生菌六神曲淀粉酶活力为(459.22±6.39) U/g,总黄酮和总多酚的含量分别提高了28.57%,20.59%。In this study,three strains of lactic acid bacteria with highβ-glucosidase enzyme activity were used for the enhanced fermentation of massa medicata fermentata,and the ratios were determined by orthogonal experiments,and the influences of fermentation time,liquid-material ratio,inoculum amount,fermentation temperature,and volume of curd block on the enhancement of massa medicata fermentata were investigated by one-way tests.Using amylase activity as the detection index,the key factors affecting the preparation process of massa medicata fermentata were screened by the Plackett-burman test,and the preparation process of massa medicata fermentata with the addition of probiotic bacteria was optimized by the Box-behnken design-response surface method.The results showed that the fermentation time was 4 d,the fermentation temperature was 32℃,the ratio of solid to liquid was 1∶1.1,and the inoculum volume was 3%.The activity of amylase was(459.22±6.39)U/g,and the contents of total flavonoids and total polyphenols were increased by 28.57%and 20.59%,respectively.

关 键 词：益生菌 六神曲 强化 响应面 工艺 

分 类 号：R283.3[医药卫生—中药学]