辣椒素和多黏菌素E协同作用对抗耐药大肠杆菌研究  

The synergistic action of capsaicin andpolymyxin E against drug-resistant Escherichia coli

作　　者：田晨宇 周鹏程 黄昕 谭杨琦 张宇龙 李基云 TIAN Chenyu;ZHOU Pengcheng;HUANG Xin;TAN Yangqi;ZHANG Yulong;LI Jiyun(College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan Province 410125,China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410125

出　　处：《中国饲料》2025年第7期44-47,53,共5页China Feed

基　　金：国家级大学生创新创业训练计划(S202310537018);湖南省教育厅资助科研项目(22B0189)。

摘　　要：本研究旨在评估中草药成分辣椒素与抗生素多黏菌素E联合应用对大肠杆菌体外抗菌效果。通过测定最小抑菌浓度(MIC)、联合抑菌指数(FICI)及生长曲线,对辣椒素、多黏菌素E单独及联合使用的抑菌作用进行了比较。结果显示:对于大肠杆菌LN175而言,辣椒素的MIC为4 mg/mL,多黏菌素E的MIC为128μg/mL,且辣椒素与多黏菌素E联合使用时表现出协同抗菌效应(FICI=0.25)。生长曲线分析进一步证明,联合应用两药后3 h内,菌液浓度显著低于单独使用任一药物组(P<0.05)。综上,辣椒素与多黏菌素E的联用能显著增强对大肠杆菌耐药菌的抑菌效果,具有潜在的抗菌应用价值。本研究为辣椒素与多黏菌素E联合抗菌策略提供了科学依据。This study aimed to evaluate the antibacterial effects of the combination of the traditional Chinese medicine component capsaicin and the antibiotic polymyxin E against Escherichia coli in vitro.By measuring the minimum inhibitory concentration(MIC),the fractional inhibitory concentration index(FICI),and growth curves,the antibacterial action of capsaicin,polymyxin E,and their combination was compared.Results showed that E.coli LN175 had an MIC of 4 mg/mL for capsaicin and 128μg/mL for polymyxin E,with the combination demonstrating a synergistic antibacterial effect(FICI=0.25).Growth curve analysis further confirmed that within 3 hours of combined drug application,the bacterial liquid concentration was significantly lower than that of groups using either drug alone(P<0.05).The study concluded that the combined use of capsaicin and polymyxin E significantly enhances the antibacterial effect against antibiotic-resistant E.coli,offering potential for antimicrobial applications.This research provided a scientific basis for the combined antimicrobial strategy of capsaicin and polymyxin E.

关 键 词：大肠杆菌 耐药性 辣椒素 多黏菌素E 中草药 

分 类 号：S852.61[农业科学—基础兽医学]