沧州市食源及人源性沙门氏菌的分子分型及耐药性分析  

Molecular typing and drug resistance of food-borne and human-borne Salmonella in Cangzhou City

作　　者：赵玲玲[1] 郑邵辉 张瑞 郭晓悦 卢俊荣[1] 王春东[1] ZHAO Lingling;ZHENG Shaohui;ZHANG Rui;GUO Xiaoyue;LU Junrong;WANG Chundong(Cangzhou Center for Disease Control and Prevention,Cangzhou,Hebei 061000,China)

机构地区：[1]沧州市疾病预防控制中心,河北沧州061000

出　　处：《华南预防医学》2025年第1期41-45,共5页South China Journal of Preventive Medicine

基　　金：沧州市科技计划自筹项目(222106004)。

摘　　要：目的 了解2021—2023年沧州市食品监测和病例监测中沙门氏菌血清型、分子分型特征及耐药情况,为控制沙门氏菌耐药株的流行提供参考。方法 采集2021—2023年沧州市食品样本和食源性疾病主动监测样本,分离沙门氏菌株,使用全自动药敏仪开展药敏试验,进行血清分型,全基因组测序进行分子分型。结果 1 258份样本中,检出沙门氏菌84株,检出率为6.7%。血清型有26种,其中鼠伤寒沙门氏菌(2.1%)、肠炎沙门氏菌(1.5%)及肯塔基沙门氏菌(0.5%)检出率较高。鼠伤寒沙门氏菌、肠炎沙门氏菌、肯塔基沙门氏菌、其他沙门氏菌分别对13、12、13、13种抗生素耐药。84株沙门氏菌总体对氨苄西林耐药最高(76.2%),其次为四环素(61.9%)和链霉素(47.6%),对多粘菌素E、替加环素均敏感(0.0%)。基于wgSNP(全基因单核苷酸多态性)的聚类分析,优势分型有2组,即ST34和ST11,存在同源菌株,但耐药谱不完全相同。结论 沧州市食源性和人源性沙门氏菌有耐药性且具有多重耐药;分子分型呈多态分布,且有明显的优势分型。相关部门应加大监测力度,对沙门氏菌耐药菌株的产生和流行起到预警作用。Objective To understand the serotypes,molecular typing characteristics,and drug resistance of Salmonella in food surveillance and case surveillance in Cangzhou City from 2021 to 2023,and to provide the reference for reducing and controlling the prevalence of drug-resistant strains of Salmonella.Methods Food samples and foodborne disease active surveillance samples were collected from 2021 to 2023 in Cangzhou City,Salmonella strains were isolated,drug sensitivity tests were carried out using an automatic drug sensitizer,diagnostic sera were serotyped,and molecular typing was performed by whole genome sequencing.Results A total of 84 strains of Salmonella were detected in 1258 samples,the detection rate was 6.7%.There were 26 serotypes,among which Salmonella typhimurium(2.1%),Salmonella enteritidis(1.5%),and Salmonella Kentucky(0.5%)had higher detection rates.Salmonella typhimurium,Salmonella enteritidis,Salmonella Kentucky,and other Salmonella were resistant to 13,12,13,and 13 kinds of antibiotics,respectively.The resistance to ampicillin was the highest in 84 strains(76.2%),followed by tetracycline(61.9%)and streptomycin(47.6%),and polymyxine E and tigacycline were the most sensitive(0.0%).Based on the cluster analysis of wgSNP(whole gene single nucleotide polymorphism),there were two dominant typing groups,namely ST34 and ST11,there were homologous strains,but the drug resistance spectrum was not exactly the same.Conclusions Food-borne and human-borne Salmonella in Cangzhou exhibit drug resistance and multidrug resistance.The molecular typing is polymorphic and demonstrates significant superiority.It is imperative for relevant departments to enhance monitoring efforts to anticipate the emergence and prevalence of drug-resistant strains of Salmonella.

关 键 词：沙门氏菌 血清型 分子分型 耐药性 

分 类 号：R115[医药卫生—公共卫生与预防医学]