Coffea arabica var.Borbon Biochemical Response to Chitosan Oligosaccharides Foliar Exposure  

作　　者：Alexis Salazar-Navarro Víctor Ruíz-Valdiviezo JoséJoya-Dávila Daniel González-Mendoza Olivia Tzintzun-Camacho Ulin Basilio-Cortes Dagoberto Duran-Hernández Henry López-Lopez Onecimo Grimaldo-Juárez 

机构地区：[1]Laboratory of Biotecnology,Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC),Nuevo León,21705,Baja California,México [2]Department of Molecular Biology,Tecnológico Nacional de México,Instituto Tecnológico de Tuxtla Gutierrez,Tuxtla Gutierrez,29050,Chiapas,México

出　　处：《Phyton-International Journal of Experimental Botany》2025年第3期641-655,共15页国际实验植物学杂志(英文)

摘　　要：The biochemical response of Coffea arabica var.Borbon to chitosan and chitosan oligosaccharides(COS)was evaluated in one-year-old plants under greenhouse conditions.COS solutions were synthesized through chemical and physical hydrolysis using acetic acid,hydrogen peroxide,and microwave irradiation.The obtained COS had an average molecular weight(Mw)of 3549.90±0.33 Daltons(Da),a deacetylation degree(DD)of 76.64±1.12%,and a polymerization degree(PD)of 18.91±0.0018.Solutions of chitosan and COS were applied to C.arabica var.Borbon at concentrations of 0.25,0.5,and 1 wt%.The experimental design was conducted using a completely randomized design with four replications.The biochemical responses assessed included soluble protein content,phenylalanine ammonia-lyase(PAL),chitinase,β-1,3-glucanase,peroxidase,catalase,and chlorophyll fluorescence.The application of COS demonstrated significant differences(α=0.05)in protein concentration,with the activity ofβ-1,3-glucanase,chitinase,and catalase being 1.5,7.5,and 3.9 times higher,respectively,while showing similar behavior to chitosan in PAL activity,both up to 4.4 times higher than the distilled water control and lower than chitosan in peroxidase activity.Treatments with chitosan yielded a higher photochemical efficiency of Photosystem II(PSII).The application of COS suggests a viable foliar alternative to active plant defense mechanisms without the risk of phytotoxicity.

关 键 词：Enzymatic activity PAL Β-1 3-GLUCANASE CHITINASE chlorophyll fluorescence photosystem II 

分 类 号：G63[文化科学—教育学]