Development and Assessment of Reference Genes for qPCR of Malus transitoria under Drought Stress  

作　　者：Ting Li Jun Xv Defang Zhang 

机构地区：[1]Qinghai Academy of Agriculture and Forestry,Qinghai University,Xining,810016,China [2]Laboratory for Research and Utilization of Germplasm Resources in Qinghai Tibet Plateau,Xining,810016,China [3]Key Laboratory of Tree Genetics and Breeding of Qinghai Plateau,National Forestry and Grassland Administration,Xining,810016,China

出　　处：《Phyton-International Journal of Experimental Botany》2025年第3期911-927,共17页国际实验植物学杂志(英文)

基　　金：supported by the Natural Science Foundation of Qinghai Province(2022-ZJ-902).

摘　　要：The use of a stable reference gene is fundamental for achieving reliable quantitative qRT-PCR (qPCR) results. Developing and evaluating the stability of reference genes is necessary for studying the molecular mechanisms of M. transitoria in response to drought stress. In this study, 18 candidate reference genes were selected from transcriptome sequencing data of M. transitoria according to their FPKM values under different drought stress degrees. Cluster-23533.34641 was identified as the most stable reference gene for M. transitoria under drought stress based on qPCR results and combined analysis of Genorm, NormFinder, BestKeeper, and Delta Ct algorithms. The reference genes identified in this research offer improved accuracy for quantifying target gene expression in both M. transitoria and Malus species under drought stress. This study could provide insights into the drought stress-related functional gene or factor in M. transitoria, even in Malus species.

关 键 词：Reference genes Malus transitoria drought stress qPCR 

分 类 号：R73[医药卫生—肿瘤]