lncRNA CASC19调节miR-410-3p/HMGB1信号轴对乳腺癌细胞增殖、侵袭和迁移的影响  

Effects of lncRNA CASC19 on proliferation,invasion and migration of breast cancer cells by regulating miR-410-3p/HMGB1 signal axis

作　　者：杨雯靖 李建梅 金鑫 张倩 贾江 YANG Wenjing;LI Jianmei;JIN Xin;ZHANG Qian;JIA Jiang(Department of Thoracic Oncology,Tianjin Cancer Hospital Qinhuangdao Hospital,Qinhuangdao,Hebei 066000,China;Department of Comprehensive Oncology,Tianjin Cancer Hospital Qinhuangdao Hospital,Qinhuangdao,Hebei 066000,China;Department of Interfere,Tianjin Cancer Hospital Qinhuangdao Hospital,Qinhuangdao,Hebei 066000,China)

机构地区：[1]天津市肿瘤医院秦皇岛医院胸部肿瘤科,河北秦皇岛066000 [2]天津市肿瘤医院秦皇岛医院综合肿瘤科,河北秦皇岛066000 [3]天津市肿瘤医院秦皇岛医院介入科,河北秦皇岛066000

出　　处：《中国优生与遗传杂志》2025年第1期45-51,共7页Chinese Journal of Birth Health & Heredity

基　　金：2021年度河北省医学科学研究项目(20210772)。

摘　　要：目的 探讨长链非编码RNA癌症易感基因19(lncRNACASC19)靶向调节miR-410-3p/高迁移率组蛋白1(HMGB1)信号轴对乳腺癌细胞增殖、侵袭和迁移的影响。方法 将乳腺癌细胞分为对照(Control)组、si-NC组、si-CASC19组、si-CASC19+inhibitor NC组、si-CASC19+miR-410-3p inhibitor组。实时定量反转录PCR(qRT-PCR)检测细胞中lncRNACASC19、miR-410-3p表达;StarBase数据库结合双荧光素酶基因检测系统验证lncRNACASC19、miR-410-3p和HMGB1之间的靶向关系;CCK-8、Transwell及细胞划痕实验检测SKBR3细胞的增殖活性、侵袭和迁移能力;Westernblot法检测各组SKBR3细胞中HMGB1蛋白表达水平。结果 与人正常乳腺上皮细胞相比,乳腺癌SKBR3细胞中lncRNA CASC19水平升高,miR-410-3p水平降低(P<0.05);si-CASC19组SKBR3细胞中lncRNA CASC19表达水平、细胞增殖活性、侵袭细胞数、划痕愈合率、HMGB1蛋白表达均降低(P<0.05),miR-410-3p表达水平升高(P<0.05);但si-lncRNACASC19与miR-410-3pinhibitor共转染细胞增殖活性、侵袭细胞数、划痕愈合率、HMGB1蛋白表达均升高(P<0.05),miR-410-3p表达水平降低(P<0.05);双荧光素酶报告基因检测结果显示miR-410-3p mimics与CASC19 WT、HMGB1 WT共转染SKBR3细胞的荧光素酶相对活性要显著低于miR-NC与CASC19 WT、HMGB1 WT共转染的细胞(P<0.05)。结论 干扰lncRNA CASC19可通过靶向上调miR-410-3p,抑制HMGB1蛋白水平,降低乳腺癌细胞的增殖活性、侵袭和迁移能力。Objective To investigate the effects of long non-coding RNA cancer susceptibility candidate 19(lncRNA CASC19) targeting the miR-410-3p/high mobility group protein 1(HMGB1) signal axis on the proliferation, invasion and migration of breast cancer cells. Methods Breast cancer cells were separated into Control group, si-NC group, si-CASC19group, si-CASC19+inhibitor NC group, and si-CASC19+miR-410-3p inhibitor group. qRT-PCR was applied to detect the expression of lncRNA CASC19 and miR-410-3p in cells. The StarBase database combined with a dual luciferase gene detection system was applied to validate the targeting relationship between lncRNA CASC19, miR-410-3p, and HMGB1. CCK-8,Transwell, and cell scratch assays were applied to detect the proliferation activity, invasion, and migration ability of SKBR3 cells. Western blot method was applied to detect the expression level of HMGB1 protein in SKBR3 cells of each group. Results Compared with human normal breast epithelial cells, the level of lncRNA CASC19 in breast cancer SKBR3 cells was higher, and the level of miR-410-3p was lower(P<0.05). The expression level of lncRNA CASC19, cell proliferation activity,number of invasive cells, scratch healing rate, and the expression of HMGB1 protein in SKBR3 cells in si-CASC19 group were all reduced(P<0.05), while the expression level of miR-410-3p was increased(P<0.05). However, the proliferation activity, invasive cell count, scratch healing rate, and HMGB1 protein expression of cells co transfected with si-lncRNA CASC19 and miR-410-3p inhibitor were all increased(P<0.05), while the expression level of miR-410-3p was decreased(P<0.05). The results of dual luciferase reporter gene detection showed that the relative activity of luciferase in SKBR3 cells co transfected with miR-410-3p mimetics and CASC19 WT, HMGB1 WT was prominently lower than that in cells co transfected with miR-NC and CASC19 WT, HMGB1 WT(P<0.05). Conclusion Interference with lncRNA CASC19 can target and up regulate miR-410-3p, inhibit HMGB1 protein level, and r

关 键 词：乳腺癌 长链非编码RNA癌症易感基因19 微小RNA-410-3p/高迁移率组蛋白1 

分 类 号：R737.9[医药卫生—肿瘤]