王不留行药用植物麦蓝菜叶片转录组测序分析  

Transcriptome sequencing and analysis of medicinal plant Gypsophila vaccaria leaf

作　　者：张超强 尉明杰 张家银 杨彬 任培荣 景小强 张勇 王恩军 ZHANG Chaoqiang;WEI Mingjie;ZHANG Jiayin;YANG Bin;REN Peirong;JING Xiaoqiang;ZHANG Yong;WANG Enjun(College of Life Sciences and Engineering,Hexi University,Zhangye Gansu 734000,China;Advantageous Biomass Resource Conversion and Utilization Technology Center in Hexi Corridor,Hexi University,Zhangye Gansu 734000,China;Key Laboratory for Biodiversity Science and Ecological Engineering of Ministry of Education,Institute of Biodiversity Science,Fudan University,Shanghai 200438,China;Department of Traditional Chinese Medicine,Minle County Traditional Chinese Medicine Hospital,Minle Gansu 734500,China;College of Agriculture and Ecological Engineering,Hexi University,Zhangye Gansu 734000,China)

机构地区：[1]河西学院生命科学与工程学院,甘肃张掖734000 [2]河西走廊优势生物质资源转化与利用技术中心,甘肃张掖734000 [3]复旦大学生物多样性与生态工程教育部重点实验室生物多样性研究所,上海200438 [4]甘肃省民乐县中医医院中医内科,甘肃民乐734500 [5]河西学院农业与生态工程学院,甘肃张掖734000

出　　处：《中兽医医药杂志》2025年第2期1-7,共7页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省自然科学基金(22JR11RG225);河西学院博士科研启动金(KYQD2022013);甘肃省科技计划项目(23CXNG0006);张掖市科技计划项目(ZY2022KY01);河西学院校长基金创新团队项目(CXTD2022011);大学生创新创业训练计划项目(202210740073,S202410740028)。

摘　　要：为获得王不留行的部分转录组信息特征,本研究基于Illumina 6000平台,对麦蓝菜叶片进行高通量测序,并组装了所有的转录本,原始数据上传NCBI数据库,登录号:PRJNA1149650。去冗余后得到99766条unigenes,在七大数据库中获得功能注释65079条,其中NR数据库60784条(占比93.40%)、SwissProt数据库46761条(占比71.85%)、EggNOG数据库57269条(占比87.99%)、KEGG数据库20073条(占比30.84%)、COG/KOG数据库57269条(占比87.99%)、Pfam数据库55514条(占比85.30%)、GO数据库33791条(占比51.92%)。bHLH、bZIP和MYB等转录因子可能与王不留行黄酮类次生代谢产物合成有关,同时,还从99766条unigenes中检测出26243个位点。转录组数据的获得丰富了麦蓝菜基因数据库,为麦蓝菜遗传图谱构建、SSR标记开发、品种选育及解析王不留行黄酮类化合物次生代谢途径和调控机制研究提供了基础数据。To obtain the transcriptomic information characteristics of Semen Vaccariae,this study conducted high-throughput sequencing of Gypsophila vaccaria leaves using the Illumina 6000 platform and assembled all transcripts.The raw data was uploaded to the NCBI database with the accession number of PRJNA1149650.A total of 99766 unigenes were generated after the removal of redundancy,of which 65079 were annotated in seven databases.They were 60784(NR:93.40%),46761(SwissProt:71.85%),57269(EggNOG:87.99%),20073(KEGG:30.84%),57269(KOG:87.99%),55514(Pfam:85.30%)and 33791(GO:51.92%).Transcription factors such as bHLH,bZIP,and MYB might be involved in the biosynthesis of flavonoids secondary metabolites.Additionally,26243 SSR loci were detected from 99766 unigenes.These results can provide basic data for the construction of genetic map,SSR marker development,variety breeding,and analysis of secondary metabolic pathways and regulatory mechanisms of flavonoids of G.vaccaria,and also enrich the G.vaccaria gene database.

关 键 词：王不留行 麦蓝菜 转录组 UNIGENE 转录因子 SSR标记 

分 类 号：S853.7[农业科学—临床兽医学]