Exploring enterovirus pathogenesis and cancer therapy potential through reverse genetics  

作　　者：Shijin Wang Qing Yu Junfeng Zhou Wanshan Yang Yonggang Wang Haoran Guo Wei Wei 

机构地区：[1]Cancer Center,The First Hospital of Jilin University,Changchun 130021 China [2]Insttute of Virology and AIDS Research,The First Hospital of Jilin University,Changchun 130021 China [3]Department of Pathology,Medical College,Yanbian University,Yanji 136200 China [4]Departnent of Dermatology,The First Hospital of Jilin University,Changchun 130021 China [5]Department of Cardiovascular Center,The First Hospital of Jilin University,Changchun 130021 China [6]Institute of Translational Medicine,Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education,The First Hospitalof jilin University,Changchun 130021 China

出　　处：《Biosafety and Health》2025年第1期74-82,共9页生物安全与健康(英文)

基　　金：supported in part by funding from the National Natural Science Foundation of China(32222005,82372226,and 82172246);the Open Project of Key Laboratory of Organ Regeneration and Transplantation,Ministry of Education,Program for JLU Science and Technology Innovative Research Team(2017TD-08);the Fundamental Research Funds for Central Universities.

摘　　要：Over the past decades,oncolytic viruses have emerged as prominent therapeutic agents with significant potential for anticancer treatment.Enteroviruses(EVs)have garnered particular attention due to their specific tropism for various types of tumor cells.The rapid advancement of reverse genetics has enabled its application in the genetic modification of enteroviruses and the investigation of viral infection mechanisms.The utilization of reverse genetics has significantly enhanced our understanding of the infection mechanisms and pathogenesis of enteroviruses,while concurrently facilitating the development of translational therapies related to these viruses.In this review,we summarize the progress in the application of reverse genetics to oncolytic enteroviruses and their potential clinical applications.Specifically,we discuss the characteristics of EVs and the applications of reverse genetics in EV research.We review the utilization of reverse genetics in mechanistic investigations of EVs and in exploring the oncolytic potential of EVs.Further,we discuss the oncolytic roles of specific EVs including EV-A71,coxsackievirus B3(CV-B3),echovirus 7(Echo-7),CV-A21,and poliovirus.Our review highlights the advances in oncolytic therapy utilizing EVs with specific tumor tropism,which holds significant potential for immunotherapy.

关 键 词：Reverse genetics Enterovirus(EV) Oncolytic virus(Ov) PATHOGENESIS Tumor immunity 

分 类 号：R373[医药卫生—病原生物学] R730.5[医药卫生—基础医学]