糙皮侧耳AQPs基因家族鉴定与表达分析  

Identification and expression analysis of the aquaporin gene family in Pleurotus ostreatus

作　　者：胡继鹏 鲍大鹏[1,2] 邹根 尚俊军 高贺宇 唐利华 HU Jipeng;BAO Dapeng;ZOU Gen;SHANG Junjun;GAO Heyu;TANG Lihua(College of Food Science,Shanghai Ocean University,Shanghai 201306;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Shanghai 201403)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院食用菌研究所农业农村部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心,上海201403

出　　处：《菌物学报》2025年第3期50-61,共12页Mycosystema

基　　金：国家自然科学基金(32172649);山东重点研发计划(2022CXGC010610)。

摘　　要：水通道蛋白(AQP)基因家族在真菌的水分运输及多种生理过程中发挥重要作用。本研究通过生物信息学方法系统鉴定糙皮侧耳Pleurotus ostreatus AQP家族成员(PoAQPs),并对其理化性质、亚细胞定位、染色体定位、系统进化关系、保守基序以及启动子顺式作用元件进行分析。同时,通过RT-qPCR检测PoAQPs基因在不同生长阶段以及水分过剩胁迫条件下的相对表达量。分析显示,糙皮侧耳基因组中共鉴定出5个PoAQPs成员,分为经典AQP和AQGP 2个亚家族,均含有MIP超家族结构域和经典的水通道蛋白三级结构特征;5个PoAQPs基因分布于4条染色体上,亚细胞定位预测结果显示,这些基因均定位于细胞膜;同一亚家族的成员具有相似的保守基序;启动子区域富含大量的激素响应和非生物胁迫相关的调控作用元件;RT-qPCR结果显示,PoAQPs基因在不同生长阶段和水分过剩胁迫条件下的表达存在显著差异,且主要在糙皮侧耳的子实体阶段以及水分过剩胁迫8h中高表达。推测PoAQPs在调控糙皮侧耳的生长发育及非生物胁迫响应中发挥作用,这一发现为深入理解糙皮侧耳的水分调控机制及抗胁迫品种的培育提供了理论支持。The aquaporin(AQP)gene family plays a crucial role in water transport and various physiological processes in fungi.In this study,the members of the AQP family in Pleurotus ostreatus(PoAQPs)were systematically identified using bioinformatics approaches,and their physicochemical properties,subcellular localization,chromosomal distribution,phylogenetic relationships,conserved motifs,and cis-regulatory elements of the promoters were analysed.The relative expression levels of PoAQPs were evaluated in various growth stages and under waterlogging stress conditions using RT-qPCR.As a result,five members of the aquaporin(PoAQPs)gene family in the genome of Pleurotus ostreatus were identified,which were categorized into two subfamilies,classical aquaporins(AQPs)and aquaglyceroporins(AQGPs).All members contained the MIP superfamily domain and exhibited the characteristic tertiary structure of classical water channel proteins.The five PoAQPs genes were distributed among four chromosomes,and subcellular localization predictions indicated that these genes were located at the cell membrane.Members within the same subfamily shared similar conserved motifs.The promoter regions were enriched with numerous regulatory elements related to hormonal responses and abiotic stress.RT-qPCR results revealed significant differences in the expression levels of PoAQPs genes in various growth stages and in response to waterlogging stress,with particularly high expression during the fruiting body stage and after 8 hours of waterlogging stress.It is suggested that PoAQPs are involved in the regulation of the growth,the development of Pleurotus ostreatus as well as the responses to abiotic stress.These findings offer valuable theoretical insights into the mechanisms of water regulation in Pleurotus ostreatus and give support to the breeding of stress-resistant varieties.

关 键 词：糙皮侧耳 水通道蛋白 基因家族 生物信息学 表达分析 

分 类 号：R73[医药卫生—肿瘤]