Saponins from Aralia taibaiensis protect against brain ischemia/reperfusion injuries by regulating the apelin/AMPK pathway  

作　　者：Zhengrong Li Yuwen Liu Kedi Liu Xingru Tao Naping Hu Wangting Li Jialin Duan 

机构地区：[1]Xi’an Key Laboratory of Stem Cell and Regenerative Medicine,Institute of Medical Research,Northwestern Polytechnical University,Xi’an 710072,China [2]TANK Medicinal Biology Institute of Xi’an,Xi’an 710065,China [3]Department of Pharmacy,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China [4]Department of Pharmacy,General Hospital of Xinjiang Production and Construction Corps,Urumqi 830092,China [5]Shanghai Minhang Collaborative Innovation Center of Northwestern Polytechnical University,Shanghai 201108,China

出　　处：《Chinese Journal of Natural Medicines》2025年第3期299-310,共12页中国天然药物(英文版)

基　　金：supported by the National Science Foundation of China(No.81903832),the Key Research and Development Plan of Shaanxi Province(No.2022SF-182),the Fundamental Research Funds for the Central Universities(No.D5000210799);Shaanxi Provincial Traditional Chinese Medicine Administration"Double Chain Integration"Middle Youth Research and Innovation Team Project(No.2022-SLRH-LJ-007);Shanghai Science and Technology Innovation Action Plan(No.22S21902600).

摘　　要：Aralia taibaiensi,widely distributed in western China,particularly in the Qinba Mountains,has been utilized as a folk medicine for treating diabetes,gastropathy,rheumatism,and cardiovascular diseases.Saponins from A.taibaiensis(sAT)have demonstrated protective effects against oxidative stress and mitochondrial dysfunction induced by ischemia/reperfusion(I/R).However,the underlying mechanisms remain unclear.In vivo,middle cerebral artery occlusion/reperfusion(MCAO/R)induced inflammatory infiltration,neuronal injury,cell apoptosis,mitochondrial dysfunction,and oxidative stress in the ischaemic penumbra,which were effectively mitigated by sAT.sAT increased the mRNA and protein expression levels of apelin and its receptor apelin/apelin receptors(ARs)both in vivo and in vitro.(Ala13)-Apelin-13(F13A)and small interfering RNA(siRNA)abolished the regulatory effects of sAT on neuroprotection mediated by adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK)/protein kinase B(Akt).Furthermore,sAT induced apelin/AR expression by simultaneously inhibiting P38 mitogen-activated protein kinase(P38 MAPK)/activating transcription factor 4(ATF4)and upregulating hypoxia-inducible factor-1α(HIF-1α).Our findings indicate that sAT regulates apelin/AR/AMPK by inhibiting P38 MAPK/ATF4 and upregulating HIF-1a,thereby suppressing oxidative stress and mitochondrial dysfunction.

关 键 词：Aralia taibaiensis Apelin/apelin receptor Stroke P38 MAPK/ATF4 HIF-1A 

分 类 号：R54[医药卫生—心血管疾病]