Discovery of fernane-type triterpenoids from Diaporthe discoidispora using genome mining and HSQC-based SMART technology  

作　　者：Yajing Wang Yongfu Li Yan Dong Chunyan Yu Chengwei Liu Chang Li Yi Sun Yuehu Pei 

机构地区：[1]Department of Medicinal Chemistry and Natural Medicine Chemistry,College of Pharmacy,Harbin Medical University,Harbin 150081,China [2]Key Laboratory for Enzyme and Enzyme-like Material Engineering of Heilongjiang,College of Life Science,Northeast Forestry University,Harbin 150040,China [3]Key Laboratory of Gut Microbiota and Pharmacogenomics of Heilongjiang Province,College of Pharmacy,Harbin Medical University,Harbin 150081,China [4]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Chinese Journal of Natural Medicines》2025年第3期368-376,共9页中国天然药物(英文版)

基　　金：supported by the Outstanding Youth Foundation of Heilongjiang Province(No.YQ2021H009).

摘　　要：In this study,we employed a combination of genome mining and heteronuclear single quantum coherence(HSQC)-based small molecule accurate recognition technology(SMART)technology to search for fernane-type triterpenoids.Initially,potential endophytic fungi were identified through genome mining.Subsequently,fine fractions containing various fernane-type triterpenoids were selected using HSQC data collection and SMART prediction.These triterpenoids were then obtained through targeted isolation and identification.Finally,their antifungal activity was evaluated.As a result,three fernane-type triterpenoids,including two novel compounds,along with two new sesquiterpenes and four known compounds were isolated from one potential strain,Diaporthe discoidispora.Their structures were elucidated through analysis of high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)and nuclear magnetic resonance(NMR)spectroscopic data.The absolute configurations were determined using single-crystal X-ray diffraction analysis and electron capture detector(ECD)analysis.Compound 3 exhibited moderate antifungal activity against Candida albicans CMCC 98001 and Aspergillus niger.

关 键 词：Fernane TRITERPENOID Genome mining SMART technology Endophytic fungi Diaporthe discoidispora 

分 类 号：O62[理学—有机化学]