miR-195-5p、CPNE1在宫颈鳞状细胞癌组织中的表达及与患者临床病理特征、预后的关系研究  

Expressions of miR-195-5p and CPNE1 in cervical squamous cell carcinoma tissues and their relationship with clinicopathological features and prognosis of patients

作　　者：崔媛[1] 王文文 田秀兰[1] CUI Yuan;WANG Wenwen;TIAN Xiuan(Department of Obstetrics and Gynecology,Beijing Tongren Hospital Affiliated to Capital Medical University,Beijing 100730,China)

机构地区：[1]首都医科大学附属北京同仁医院妇产科,100730

出　　处：《浙江医学》2025年第6期584-589,共6页Zhejiang Medical Journal

基　　金：北京同仁医院基金资助项目(京同医字[2021]76号)。

摘　　要：目的观测微小RNA-195-5p(miR-195-5p)、钙依赖膜结合蛋白Copine蛋白1(CPNE1)在宫颈鳞状细胞癌(CSCC)组织中的表达情况,分析其与患者临床病理特征、预后的关系。方法回顾性选取2015年6月至2020年6月在首都医科大学附属北京同仁医院行手术治疗的CSCC患者102例,留取术中切除的CSCC组织和配对的正常癌旁组织为研究标本。采用实时荧光定量聚合酶链反应检测CSCC组织和癌旁组织miR-195-5p、CPNE1 mRNA表达水平,分析其与患者临床病理特征的关系。随访3年,以CSCC组织miR-195-5p、CPNE1 mRNA表达水平平均值为截点将患者分为高表达(≥平均值)患者和低表达(<平均值)患者,采用Kaplan-Meier生存曲线分析CSCC组织miR-195-5p、CPNE1 mRNA表达水平与患者预后的关系;采用Cox回归分析CSCC患者预后的影响因素。结果CSCC组织miR-195-5p表达水平低于癌旁组织(P<0.05),CPNE1 mRNA表达水平高于癌旁组织(P<0.05)。FIGO分期Ⅰ~Ⅱ期、中高分化、无淋巴结转移患者CSCC组织miR-195-5p表达水平分别高于Ⅲ~Ⅳ期、低分化、有淋巴结转移患者,CPNE1 mRNA表达水平分别低于Ⅲ~Ⅳ期患者低分化、有淋巴结转移患者,差异均有统计学意义(均P<0.05)。随访期间失访2例,死亡34例,miR-195-5高表达患者的3年生存率为76.92%(40/52),高于miR-195-5低表达患者的54.17%(26/48),差异有统计学意义(P<0.05);CPNE1 mRNA高表达患者的3年生存率为52.83%(28/53),低于CPNE1 mRNA高表达患者的80.85%(38/47),差异有统计学意义(P<0.05)。低分化、淋巴结转移和CPNE1高表达均是CSCC患者预后不良的独立危险因素(均P<0.05),miR-195-5p高表达是预后的保护因素(P<0.05)。结论miR-195-5p在CSCC组织中呈低表达,CPNE则呈高表达,与患者肿瘤低分化、淋巴结转移及不良预后的发生有关。Objective To investigate the expressions of microRNA-195-5p(miR-195-5p)and calcium-dependent membrane binding protein Copine protein 1(CPNE1)in cervical squamous cell carcinoma(CSCC)tissues,and to analyze their relationship with clinicopathological features and prognosis of patients.Methods A total of 102 CSCC patients who underwent surgical treatment at Beijing Tongren Hospital Affiliated to Capital Medical University from June 2015 to June 2020 were retrospectively selected,and CSCC tissues removed during operation and matched normal para-cancer tissues were retained as study specimens.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression levels of miR-195-5p and CPNE1 mRNA in CSCC tissues and adjacent tissues,and toanalyze their relationship with clinicopathological characteristics of patients.After 3 years of follow-up,patients were divided into high expression(≥average)and low expression(<average)groups based on the mean expression levels of miR-195-5p and CPNE1 mRNA in CSCC tissues as the cut-off point.Kaplan-Meier survival curve was used to analyze the relationship ofexpression levels of miR-195-5p and CPNE1 mRNA in CSCC tissues with the prognosis of patients.Cox regression was used to analyze the prognostic factors for CSCC patients.Results The expression level of miR-195-5p in CSCC tissues was lower than that of adjacent tissues(P<0.05),while the expression level of CPNE1 mRNA in CSCC tissues was higher than that of adjacent tissues(P<0.05).The expression level of miR-195-5p in CSCC tissues of patients with Federation of Gynecology and Obstetrics(FIGO)stage Ⅰ-Ⅱ,moderate-high differentiation and no lymph node metastasis was higher than that of patients with stage Ⅲ-Ⅳ,low differentiated and lymph node metastasis,respectively,and the expression level of CPNE1 mRNA was lower than that of patients with stage Ⅲ-Ⅳ,poorly differentiated and lymph node metastasis,respectively,with statistically significant differences(all P<0.05).Two cases were lost to follo

关 键 词：宫颈鳞状细胞癌 微小RNA-195-5p 钙依赖膜结合蛋白Copine蛋白1 病理特征 预后 

分 类 号：R73[医药卫生—肿瘤]