补脾养心方改善睡眠的作用机制研究和安全性考察  

Study on the Mechanism of Action and Safety Investigation of Bupi Yangxin Decoction to(补脾养心方)Improve Sleep

作　　者：热依拉·吐尔逊 高鑫 王淳[1] 乐娜 刘冰清 廖艳[1] TUERXUN Reyila;GAO Xin;WANG Chun;YUE Na;LIU Bingqing;LIAO Yan(Beijing University of Chinese Medicine,Beijing 102401,China)

机构地区：[1]北京中医药大学,北京102401

出　　处：《辽宁中医药大学学报》2025年第4期23-27,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家重点研发计划项目(2018YFC1706800)。

摘　　要：目的探讨补脾养心方改善睡眠作用及可能机制,并初步考察其安全性。方法采用补脾养心方低(1.5 g/kg)、中(3 g/kg)、高(6 g/kg)剂量和阳性对照人参归脾丸(5 g/kg)每天进行小鼠灌胃,记录小鼠体质量变化,30 d后检测各组小鼠胸腺、脾脏指数和血清中谷丙转氨酶(alanine transaminase,ALT)、碱性磷酸酶(alkaline phosphatase,ALP)、谷草转氨酶(aspartate Transaminase,AST)、乳酸脱氢酶(lactate dehydrogenase,LDH)和肌酸激酶(creatine phosphokinase,CK)含量,HE染色法分析肝、脑组织病理变化,并通过四项睡眠实验观察小鼠睡眠改变。结果与空白对照组相比,补脾养心方未引起小鼠直接睡眠,但可延长戊巴比妥钠睡眠时间、缩短巴比妥钠睡眠潜伏期实验、降低戊巴比妥钠阈下剂量(P<0.05);给药各组小鼠体质量明显增加,胸腺、脾脏指数升高,血清CK、LDH含量下降,脑组织和肝脏组织未发现明显病理改变。结论实验表明补脾养心方具有改善睡眠的功效,其机制可能与调控炎症因子和睡眠-觉醒周期有关,且安全性较好。Objective To explore the effect and possible mechanism of Bupi Yangxin Decoction(补脾养心方)to improve sleep,and preliminarily investigate its safety.Methods The low(1.5 g/kg),medium(3 g/kg)and high(6 g/kg)doses of Bupi Yangxin Decoction and the positive control Reshen Guipi Pills Pill(人参归脾丸,5 g/kg)were administered to mice by gavage every day,and the changes in body weight of the mice were recorded.30 days later,the thymic and splenic indices of mice of each group and the serum levels of alanine transaminase(ALT),alkaline phosphatase(ALP),aspartate transaminase(AST),lactate dehydrogenase(LDH)and creatine phosphokinase(CK)were measured,dehydrogenase(LDH)and creatine kinase(CK)content,HE staining method was used to analyze the histopathological changes in liver and brain tissues,and sleep changes in mice were observed by four sleep experiments.Results Compared with the blank control group,Bupi Yangxin Decoction did not cause direct sleep in mice,but it could prolong the sleep time of sodium pentobarbital,shorten the sleep latency experiment of sodium barbiturate,and reduce the subthreshold dose of sodium pentobarbital(P<0.05);the body weight of the mice in the groups of administration increased significantly,the indexes of the thymus and the spleen were elevated,the contents of serum CK and LDH were decreased,and no obvious pathological changes were detected in the brain tissues and the liver tissues.Conclusion This experiment demonstrated that Bupi Yangxin Decoction has the efficacy of improving sleep,and its mechanism may be related to the modulation of inflammatory factors and the sleep-wake cycle,and the safety is good.

关 键 词：补脾养心方 改善睡眠 辨证保健 

分 类 号：R285.5[医药卫生—中药学]