狼尾草属叶绿体基因组特征与分子标记开发  

Chloroplast genome characteristics and molecular marker development of Pennisetum

作　　者：江转转 陈红 鲍红艳 代雨童 JIANG Zhuanzhuan;CHEN Hong;BAO Hongyan;DAI Yutong(College of Life Science/The Province Key Laboratory of the Biodiversity Study and Ecology Conservation,Anqing Normal University,Anqing 246133,Anhui,China)

机构地区：[1]安庆师范大学生命科学学院/皖西南生物多样性研究与生态保护安徽省重点实验室,安徽安庆246133

出　　处：《浙江农林大学学报》2025年第2期365-372,共8页Journal of Zhejiang A&F University

基　　金：武汉大学杂交水稻全国重点实验室开放课题(KF202204);安徽省高等学校科学研究项目(2022AH051034,2022AH051048);安徽省中青年教师行动培养计划(JWFX2023027);安徽省大学生创新创业训练项目(S202310372079)。

摘　　要：【目的】利用4种已发布的狼尾草属Pennisetum植物叶绿体基因序列,分析其叶绿体基因组特征并开发相应的分子标记,为狼尾草属植物的进化及系统发育关系提供新的观点。【方法】基于美国国家生物技术信息中心(NCBI)数据库已发布的4种狼尾草属叶绿体完整基因组序列,利用生物信息学手段分析其重复序列、密码子偏好性、多态性、共线性并开发简单重复序列(SSR)分子标记。【结果】4种狼尾草属植物的叶绿体基因组长度及GC含量都较为接近且密码子偏倚程度不高;边界扩张分析显示各区域间收缩不显著;共线性分析表明4种狼尾草植物的种间共线性较强;基于叶绿体基因组序列的系统发育分析表明白草P.flaccidum和御谷P.glaucum、象草P.purpureum和狼尾草P.alopecuroides的亲缘关系最近。5对SSR分子标记在狼尾草属植物中有较高的实用性,引物SSR2是御谷和狼尾草的特异性引物,引物SSR5是御谷的特异性引物。SSR1、SSR3和SSR4在4种狼尾草属植物中显示出较高的保守性,SSR1的种间保守性最高。【结论】4种狼尾草叶绿体基因组较为保守,且白草与御谷、象草与狼尾草的亲缘关系最为接近,同时引物SSR2是御谷和狼尾草的特异性引物,引物SSR5是御谷的特异性引物。[Objective]The objective is to analyze the chloroplast genome characteristics of 4 published plants in the genus Pennisetum,and to develop corresponding molecular markers.This will provide new insights into the evolutionary and phylogenetic relationships of Pennisetum.[Method]Bioinformatics methods were used to analyze the complete chloroplast genome sequences of 4 species of Pennisetum published in the NCBI database.The analysis included repeat sequences,codon preferences,polymorphisms,collinearity,and development of simple sequence repeat(SSR)molecular markers.[Result]The chloroplast genome length and GC content of the 4 species of Pennisetum were relatively similar,with a low degree of codon shift.Boundary expansion analysis indicated no significant contraction between different regions.Collinearity analysis showed strong inter-species collinearity among the 4 species of Pennisetum.Phylogenetic analysis based on chloroplast genome sequences revealed that P.flaccidum had the closest phylogenetic relationship with P.glaucum,P.purpureum,and P.alopecuroides.5 pairs of SSR molecular markers had high practicality in plant species within the genus Pennisetum.Specifically,primer SSR2 was expressed in both P.flaccidium and P.alopecuroides;while primer SSR5 was specifically expressed in P.flaccidum.Additionally,SSR1,SSR3,and SSR4 showed high conservatism among all four species in this genus.[Conclusion]This study concludes that the chloroplast genomes of these 4 species are relatively conserved;furthermore,it confirms that genetic relationship between P.flaccidium is closest to those found in P.flaccidum,P.purpureum as well as P.alopecuroides.SSR2 molecular markers are specifically expressed in both P.flaccidium and P.alopecuroides,while SSR5 molecular markers are specifically expressed in P.flaccidum.

关 键 词：狼尾草属 叶绿体基因组 分子标记 简单重复序列(SSR) 

分 类 号：Q753[生物学—分子生物学]