基于微流控的仿生水凝胶支架用于食管肿瘤诊疗的研究进展  

Research Progress of Biomimetic Hydrogel Scaffold Based on Microfluidic for the Diagnosis and Treatment of Esophageal Tumors

作　　者：郝思妤 李佳怡 喻莹 史嘉铭 王洛伟[2] 单探幽[1] 石林林 高社干[1] HAO Siyu;LI Jiayi;YU Ying;SHI Jiaming;WANG Luowei;SHAN Tanyou;SHI Linlin;GAO Shegan(Henan Key Laboratory of Microbiology and Esophageal Cancer Prevention and Treatment,College of Basic Medicine and Forensic Medicine,Henan University of Science and Technology,Henan Key Laboratory of Tumor Epigenetics,Cancer Hospital,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang,China,471023;Department of Gastroenterology,The First Affiliated Hospital of Naval Medical University,Shanghai,China,200433)

机构地区：[1]河南科技大学基础医学与法医学院,河南省微生物与食管癌防治重点实验室,河南省肿瘤表观遗传学重点实验室,河南科技大学第一附属医院,肿瘤医院,洛阳471023 [2]海军军医大学(第二军医大学)第一附属医院消化内科,上海200433

出　　处：《食管疾病》2025年第1期21-27,共7页Journal of Esophageal Diseases

基　　金：河南省中原英才计划(育才系列);河南省医学科技攻关省部共建重点项目(SBGJ202102199);洛阳市社会发展公益专项医疗卫生重点项目(2302016A)。

摘　　要：水凝胶可以模拟细胞外基质,维持包封细胞生物活性,从而在体外建立肿瘤细胞三维培养的微环境,构建肿瘤仿生器官。用水凝胶包封药物可以同时递送亲水性和疏水性药物,结合微流控对水凝胶精确操控和分散,可以制造不同形貌的均质功能性颗粒和连续单相或多相的层状纤维。为制造更为复杂的几何形状,3D生物打印已经成为一种多用途和可靠的工具,利用微流控3D打印可以制造不同结构的支架,移植用于肿瘤组织切除后病损部位的机械支持和物理修复,促进健康组织的再生和功能重建。本文对水凝胶性能进行了讨论,系统介绍了微流控水凝胶的制备、主要原理和相关特性,阐述了微流控水凝胶在肿瘤细胞包封和化疗药物输送方面的应用,综述了微流控水凝胶在肿瘤治疗基础上的组织工程学意义和应用前景。Among the array of medical biomaterials,hydrogels emerge as particularly noteworthy.Constituting three-dimensional networks of hydrophilic polymers,they are typically chemically or physically cross-linked and exhibit biocompatibility.With their elevated water content and resemblance to native human tissues in chemical composition and mechanical attributes,hydrogels can emulate the extracellular matrix,preserving the biological vitality of encapsulated cells.This capability enables the establishment of conducive microenvironments for the in vitro three-dimensional culture of cancer cells and the fabrication of tumor mimetic constructs.Moreover,hydrogels afford the simultaneous delivery of hydrophilic and hydrophobic drugs.Integrated with microfluidic control,hydrogels can be precisely manipulated and dispersed,yielding uniform functional particles of varied morphologies and continuous single-phase or multiphase layered fibers.For the fabrication of intricate geometries,3D bioprinting has emerged as a versatile and dependable tool.Leveraging microfluidic 3D printing,scaffolds with diverse structures can be manufactured,facilitating mechanical support and physical restoration post-tumor tissue resection while fostering the regeneration and functional reconstruction of healthy tissues.This manuscript begins with a discussion on the properties of hydrogels,followed by an exploration of the preparation,underlying principles,and associated attributes of microfluidic hydrogels.Furthermore,emphasis is placed on the application of microfluidic hydrogels in encapsulating cancer cells and delivering chemotherapy drugs.Ultimately,the tissue engineering implications and future prospects of microfluidic hydrogels in the context of tumor therapy are comprehensively reviewed.

关 键 词：食管癌 微流控 水凝胶 综合治疗 

分 类 号：R735.1[医药卫生—肿瘤]