一种多功能仿生纳米颗粒对食管癌细胞的作用研究  

Study on the Impact of a Multifunctional Biomimetic Nanoparticle on Esophageal Cancer Cells

作　　者：段智康 肖倩文 杨玉坤 张泽群 姚俊[1] DUAN Zhikang;XIAO Qianwen;YANG Yukun;ZHANG Zequn;YAO Jun(The First Affiliated Hospital of Henan University of Science and Technology,Luoyang,China,471023)

机构地区：[1]河南科技大学临床医学院,河南科技大学第一附属医院,洛阳471023

出　　处：《食管疾病》2025年第1期28-34,共7页Journal of Esophageal Diseases

摘　　要：目的合成一种食管癌细胞膜包裹的仿生纳米颗粒,探究其光热效应及对食管癌细胞的作用。方法经脂质体挤压器将食管癌细胞膜包裹于纳米核心表面,合成纳米颗粒CCM@PLGA-PEG-OXA-ICG(CCM@POI NPs)。分析纳米颗粒的形貌、粒径、Zeta电位等理化特征、观察其在808 nm近红外激光照射下的光热效应及温度稳定性。研究其对食管癌细胞增殖、迁移及侵袭能力的影响。结果成功制备了CCM@POI NPs纳米颗粒,在808 nm近红外激光照射下能迅速升温且具有良好的光热循环性,联合光热治疗后能明显抑制食管癌细胞的增殖、迁移及侵袭能力。结论CCM@POI NPs仿生纳米颗粒联合光热疗法可增强抗肿瘤作用,为食管癌临床治疗提供新思路。Objective To fabricate a biomimetic nanoparticle,that is enveloped by the membrane of tumor cells and to explore the photothermal effect and its impact on esophageal cancer cells.Methods Encapsulation of esophageal cancer cell membrane onto nano core surface using liposome extruder to synthesize nanoparticles,CCM@PLGA-PEG-OXA-ICG(CCM@POI NPs).The morphology,particle size,Zeta potential,and other physical and chemical characteristics of this nanoparticle were analyzed.Additionally,the photothermal effect and temperature stability under 808nm near-infrared laser irradiation were investigated.Its impact on the proliferation,migration,and invasion ability of esophageal cancer cells.was studied.Results The CCM@POI NPs nanoparticles were successfully synthesized,which exhibit rapid heating capabilities upon 808nm near-infrared laser irradiation and demonstrate excellent photothermal cycling performance.The combined application of photothermal therapy significantly suppresses the proliferation,migration,and invasion of esophageal cancer cells.Conclusion The combination of CCM@POI NPs biomimetic nanoparticles and photothermal therapy can enhance anti-tumor effects and provide new ideas for the clinical treatment of esophageal cancer.

关 键 词：纳米颗粒 食管癌 光热治疗 药物载体 仿生微纳技术 

分 类 号：R73-362[医药卫生—肿瘤]