TMEM33在肺腺癌的表达特征及临床病理特征  

Expression Characteristics and Clinical Pathological Features of TMEM33 in Lung Adenocarcinoma

作　　者：楼海均 张振宇 苏热彦·阿力木江 孟·孟根 陈航 乌都木丽 LOU Haijun;ZHANG Zhenyu;SUREYAN Alimujiang;MENG Menggen;CHEN Hang;WUDU Muli(Department of Pathology,Basic Medical College,Xinjiang Medical University,Urumqi Xinjiang 830017;Department of Clinical Laboratory,The Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi Xinjiang 830017,China)

机构地区：[1]新疆医科大学基础医学院病理教研室,新疆乌鲁木齐830017 [2]新疆医科大学第五附属医院检验科,新疆乌鲁木齐830017

出　　处：《昆明医科大学学报》2025年第3期19-26,共8页Journal of Kunming Medical University

基　　金：新疆维吾尔自治区重点研发计划项目(2022B03019-1-1);新疆医科大学大学生创新创业项目(X202310760005)。

摘　　要：目的 本研究旨在探讨跨膜蛋白33(transmembrane protein 33,TMEM33)在肺腺癌中的表达模式,并分析其与临床病理特征的相关性。方法 利用公共数据库(如TCGA和GEO)和生物信息学工具对TMEM33在肺癌中的表达数据进行了分析。在4种细胞系中通过免疫印迹技术和实时定量PCR检测了TMEM33的蛋白和m RNA表达水平。还通过细胞免疫荧光和免疫组织化学技术在肺腺癌组织和正常肺组织中评估了TMEM33的表达和定位。结果 生物信息学分析显示,TMEM33在肺腺癌中的表达水平高于正常肺组织(P <0.05),TMEM33与SLC30A9的表达具有相关性(P <0.0001)。Logistic回归分析显示,TMEM33的表达水平与T分期相关(OR=0.48,P=0.044)。实验结果显示,在肺腺癌细胞系中,TMEM33的蛋白(P <0.01)和m RNA(P <0.001)表达水平都高于正常肺上皮细胞。同样,在肺腺癌组织中,TMEM33的蛋白(P <0.05)和m RNA(P <0.01)表达水平都高于癌旁组织。免疫组织化学技术揭示了TMEM33在肺腺癌组织中高表达。结论 TMEM33在肺腺癌中高表达,与恶性程度和T分期相关,有望成为肺腺癌预后评估和治疗的潜在靶点。Objective This study aimed to explore the expression pattern of transmembrane protein 33(TMEM33)in lung adenocarcinoma and its correlation with clinical pathological features.Methods Bioinformatics tools and public databases(e.g.,TCGA and GEO)were used to analyze TMEM33 expression data in lung cancer.Then,immunoblotting and real-time quantitative PCR were performed to detect TMEM33 protein and mRNA levels in four cell lines.Immunofluorescence and immunohistochemistry were also used to assess TMEM33 expression and localization in lung adenocarcinoma and normal lung tissue samples.Results Bioinformatics analysis revealed higher TMEM33 expression in lung adenocarcinoma than in normal lung tissue(P<0.05)and a significant correlation between TMEM33 and SLC30A9 expression(P<0.0001).Logistic regression analysis indicated an association between TMEM33 expression and tumor T stage(OR=0.48,P=0.044).Experimental results showed higher TMEM33 protein(P<0.01)and mRNA(P<0.001)levels in lung adenocarcinoma cell lines than in normal lung epithelial cells.Similarly,TMEM33 protein(P<0.05)and mRNA(P<0.01)levels were higher in lung adenocarcinoma tissues than in adjacent tissues.Immunohistochemistry confirmed high TMEM33 expression in lung adenocarcinoma tissues.Conclusion TMEM33 is highly expressed in lung adenocarcinoma,associated with malignancy and T stage,and may be a potential prognostic and therapeutic target.

关 键 词：肺腺癌 TMEM33 生物标志物 临床病理特征 生物信息学分析 

分 类 号：R36[医药卫生—病理学]