一氧化氮检测荧光探针在疾病诊断中的应用进展  

Research progress of fluorescent probes for nitric oxide detection in disease visualization

作　　者：冯永威 许佑君[1] 张青扬 胡海宇 

机构地区：[1]沈阳药科大学制药工程学院,辽宁110016 [2]中国医学科学院北京协和医学院药物研究所合成药物化学研究室,北京100050

出　　处：《中国医药生物技术》2025年第2期181-189,共9页Chinese Medicinal Biotechnology

基　　金：北京市自然科学基金面上项目(2242025)。

摘　　要：一氧化氮(NO)作为重要的气体信号分子,与人类多种疾病紧密相关,NO浓度的实时监测和定量分析在疾病机制研究中具有重要意义。荧光探针因其高灵敏度、选择性、时空分辨率、非侵入性及操作便捷性,已成为NO检测的重要工具。然而,开发同时具备高选择性、高灵敏度、低荧光背景干扰、低细胞毒性可以用于NO实时定量检测的荧光探针仍面临诸多挑战。本综述全面回顾了近五年来NO响应型荧光探针的研究进展,按疾病类型进行分类,重点讨论了探针设计策略、NO响应机制以及其在生物成像和疾病实时监测中的应用潜力。Nitric oxide(NO)is a key gaseous signaling molecule involved in various human diseases,and real-time monitoring of its levels is crucial for understanding disease mechanisms.Fluorescent probes have become invaluable tools for detecting NO due to their high sensitivity,selectivity,temporal-spatial resolution,non-invasive nature,and convenience of operation.However,there are still many challenges in developing fluorescent probes that can be used for real-time quantification of NO with high selectivity,high sensitivity,low fluorescence background,and minimal cytotoxicity.This review provides a comprehensive overview of research progress of NO-responsive fluorescent probes in the past five years,classifying them by disease type,focusing on design strategies,NO response mechanisms,

关 键 词：一氧化氮 荧光探针 疾病诊断 可视化 实时检测 

分 类 号：Q503[生物学—生物化学] Q657.3