UBAP2L在结直肠癌中的表达及其意义  

The expression and significance of ubiquitin-associated protein 2-like in colorectal cancer

作　　者：徐昌[1,2] 吴箫 王茂凡 张翔雁 张照龙 XU Chang;WU Xiao;WANG Maofan;ZHANG Xiangyan;ZHANG Zhaolong(School of Basic Medicine,Qingdao University,Qingdao 266000,China)

机构地区：[1]青岛大学基础医学院,山东青岛266000 [2]青岛大学附属医院放疗技术中心 [3]青岛市妇女儿童医院 [4]青岛大学附属医院病理科 [5]青岛大学附属医院介入医学科

出　　处：《青岛大学学报(医学版)》2025年第1期19-23,共5页Journal of Qingdao University(Medical Sciences)

基　　金：山东省自然科学基金项目(ZR2021QH018)。

摘　　要：目的探讨泛素化相关蛋白2(UBAP2L)在结直肠癌(CRC)组织中的表达及其对CRC细胞增殖的影响。方法收集2015—2019年在青岛大学附属医院接受手术治疗的248例Ⅰ~Ⅲ期原发性CRC病人的癌和癌旁组织,采用免疫组化方法检测UBAP2L的表达。构建UBAP2L过表达和敲除的CRC细胞系,应用CCK8试剂盒检测UBAP2L对CRC细胞增殖的影响。结果CRC组织中UBAP2L的表达水平显著高于癌旁组织,差异具有统计学意义(P=0.01)。UBAP2L表达与肿瘤部位、肿瘤浸润程度有关(χ^(2)=17.82、39.45,P<0.05)。UBAP2L高表达病人无病生存期较低表达病人更短(χ^(2)=7.32,P<0.01)。敲除UBAP2L可以抑制CRC细胞增殖(F=38.97,P<0.01)。结论UBAP2L在CRC组织中高表达。UBAP2L可能成为评估CRC发生发展的分子标志物,并有望成为CRC的分子治疗靶点。Objective To investigate the expression of ubiquitin-associated protein 2-like(UBAP2L)in colorectal cancer(CRC)tissue and its effect on the proliferation of CRC cells.Methods Cancer tissue samples and paracancerous tissue samples were collected from 248 patients with stageⅠ-Ⅲprimary CRC who underwent surgical treatment in The Affiliated Hospital of Qingdao University from 2015 to 2019,and immunohistochemistry was used to measure the expression of UBAP2L.The CRC cell line with UBAP2L overexpression or knockdown was established,and CCK8 assay was used to observe the effect of UBAP2L on the proliferation of colorectal cancer cells.Results The expression level of UBAP2L in CRC tissue was significantly higher than that in paracancerous tissue(P=0.01).The expression of UBAP2L was associated with tumor location and tumor infiltration(χ^(2)=17.82,39.45,P<0.05).The patients with high UBAP2L expression had a shorter disease-free vival than those with low expression(χ^(2)=7.32,P<0.01).UBAP2L knockdown could inhibit the proliferation of CRC cells(F=38.97,P<0.01).Conclusion UBAP2L is highly expressed in colorectal cancer tissue.UBAP2L may become a molecular marker for evaluating the development and progression of colorectal cancer,and it is expected to become a molecular therapeutic target for CRC.

关 键 词：结直肠肿瘤 泛素化蛋白 病理学 临床 免疫组织化学 细胞增殖 

分 类 号：R735.34[医药卫生—肿瘤] R341[医药卫生—临床医学]