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作 者:姜玉榕 马丽 张维 李青飞 栾军 JIANG Yu-Rong;MA Li;ZHANG Wei;LI Qing-Fei;LUAN Jun(National Light Industry Food Quality Supervision and Inspection Nanjing Station,Nanjing 210024,China;Animal,Plant and Food Inspection Center,Nanjing Customs,Nanjing 210019,China)
机构地区:[1]国家轻工业食品质量监督检测南京站,南京210024 [2]南京海关动植物与食品检测中心,南京210019
出 处:《食品安全质量检测学报》2025年第8期261-266,共6页Journal of Food Safety and Quality
基 金:南京海关科研项目(2025KJ30)。
摘 要:目的研究胶体金免疫层析法检测肉制品中动物源性成分的准确性及可行性。方法利用该快速检测试剂盒检测肉制品中动物源性成分,对样品中8种动物源成分的灵敏度、特异性、掺假模拟、假阳性率、假阴性率进行相关实验,并以实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction,QPCR)为参比对照。结果该快速检测试剂盒的灵敏度对牛、鸡、鸭、鹅的检出限可达到0.1%,对猪、羊、马、驴的检出限可达到0.01%;掺假模拟中猪、牛、羊、鸡、鸭、鹅、马、驴的检出限均可达到0.5%,同时与其他非本物种通道检测时均无交叉反应,特异性较好。结论本研究建立的胶体金免疫层析法适用于肉制品中动物源性的现场快速筛查和检测。Objective To study the accuracy and feasibility of colloidal gold immunochromatography for the detection of animal-derived components in meat products.Methods The rapid detection kit was used to detect the animal-derived components in meat products.The sensitivity,specificity,adulteration simulation,false positive rate and false negative rate of 8 kinds of animal-derived components were tested,and quantitative real-time polymerase chain reaction(QPCR)was used as a reference.Results The limits of detection of the rapid detection kit could reach 0.1%for cattle,chicken,duck and goose,and 0.01%for pig,sheep,horse and donkey.The limits of detection of pig,cattle,sheep,chicken,duck,goose,horse and donkey in the adulterated simulation could reach 0.5%,and there was no cross-reaction with other non-species channel detection,and the specificity was good.Conclusion The colloidal gold immunochromatography method established in this study is suitable for the rapid screening and detection of animal origin in meat products.
关 键 词:胶体金免疫层析 快速检测 动物源成分 实时荧光定量聚合酶链式反应
分 类 号:TS2[轻工技术与工程—食品科学与工程]
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