基于代谢组学和转录组学揭示射干叶基部颜色的形成机制  

To reveal the mechanism of color formation at the base of Belamcanda chinensisthrough integrative analysis of metabolomics and transcriptomics

作　　者：马淏辰 沈弋清 吴王琪 龙婷 汪文杰 MA Hao-chen;SHEN Yi-qing;WU Wang-qi;LONG Ting;WANG Wen-jie(School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《时珍国医国药》2025年第4期751-759,共9页Lishizhen Medicine and Materia Medica Research

基　　金：刘合钢全国名老中医药专家传承工作室(国中医药人教函【2022】75号);湖北卫尔康现代中药有限公司研究生工作站(湖北中医药大学中医校【2022】125号)。

摘　　要：目的解释两品系射干叶基部颜色差异背后的分子机制。方法通过代谢组学分析,各筛选出了Malvidin 3-O-glu⁃coside、Malvin、Delphinidin 3-glucoside等15种代谢物作为射干叶基颜色的潜在标记物。通过转录组分析,发现Bc4CL1,Bc4CL2,Bc4CL3,Bc4CL4,Bc4CL5,BcADT,BcAROF1,BcAROF2,BcC3H,BcCCOM,BcCHI,BcCHS,BcCOMT,BcCSE,BcHCT1,BcHCT2,BcPAL3,BcPAL5,BcPAL8,BcPAL9,BcPER10,BcPER11,BcPER2,BcPER5,BcREF和BcTYNA2等26个基因对射干叶基花青素的生物合成有重要影响。结果高含量的花青素是射干叶基形成红色的原因。通过对代谢组学和转录组学数据的综合分析,确定了12个基因BcADT,BcPAL3,BcPAL5,BcPAL8,BcPAL9,Bc4CL1,Bc4CL2,Bc4CL3,Bc4CL4,Bc4CL5,BcCHS和BcCHI,它们可能对射干叶基红色的形成起重要作用。结论在射干叶基部红色的形成中,有12个关键基因BcADT,BcPAL3,BcPAL5,BcPAL8,BcPAL9,Bc4CL1,Bc4CL2,Bc4CL3,Bc4CL4,Bc4CL5,BcCHS和BcCHI,此外柚皮素Narin⁃genin和Naringenin-7-O-glucoside是射干叶基花青素积累的重要中间体。Objective To interpret the molecular mechanism behind the color differences between the bases of two types of Belamcanda chinensis(L.)Redouté(B.chinensis).Methods Through metabolomics analysis,15 metabolites like malvidin 3-O-glucoside,malvin,delphinidin 3-glucosid were screened out as potential markers for the color of the base of B.chinensis.Through transcriptomic analysis,it was found that 26 genes,including Bc4CL1,Bc4CL2,Bc4CL3,Bc4CL4,Bc4CL5,BcADT,BcAROF1,BcAROF2,BcC3H,BcCCOM,BcCHI,BcCHS,BcCOMT,BcCSE,BcHCT1,BcHCT2,BcPAL3,BcPAL5,BcPAL8,BcPAL9,BcPER10,BcPER11,BcPER2,BcPER5,BcREF and BcTYNA2,had significant effects on the biosynthesis of anthocyanins in the base of B.chinensis.Results The high content of anthocyanin was the reason for the formation of red at the base of B.chinensis.Through the com⁃prehensive analysis of metabolomics and transcriptomics,12 genes,including BcADT,BcPAL3,BcPAL5,BcPAL8,BcPAL9,Bc4CL1,Bc4CL2,Bc4CL3,Bc4CL4,Bc4CL5,BcCHS and BcCHI,were identified to play an important role in the formation of red at the base of B.chinensis.Conclusion There are 12 key genes,including BcADT,BcPAL3,BcPAL5,BcPAL8,BcPAL9,Bc4CL1,Bc4CL2,Bc4CL3,Bc4CL4,Bc4CL5,BcCHS and BcCHI,in the formation of red leaf base of B.chinensis.In addition,L-Phenylalanine,Cin⁃namic acid,naringenin and naringenin chalcone are important intermediates for anthocyanin accumulation in the leaf base of B.chinensis.

关 键 词：射干 代谢组 转录组 花青素 叶基部 植物色素 

分 类 号：S567[农业科学—中草药栽培]