Mapping subcellular RNA localization with proximity labeling  

作　　者：Jiapeng Liu Binglin Zhong Shuojun Li Shuo Han 

机构地区：[1]Key Laboratory of RNA Innovation,Science and Engineering,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Acta Biochimica et Biophysica Sinica》2025年第1期101-107,共7页生物化学与生物物理学报(英文版)

基　　金：supported by the grants from the National Key R&D Program of China(Nos.2023YFA1801300,and 2022YFA1304500);the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB0570000);the National Natural Science Foundation of China(Nos.92368102,and 22377126);Shanghai Municipal Science and Technology Major Project。

摘　　要：The subcellular localization of RNA is critical to a variety of physiological and pathological processes.Dissecting the spatiotemporal regulation of the transcriptome is key to understanding cell function and fate.However,it remains challenging to effectively enrich and catalogue RNAs from various subcellular structures using traditional approaches.In recent years,proximity labeling has emerged as an alternative strategy for efficient isolation and purification of RNA from these intricate subcellular compartments.This review focuses on examining RNA-related proximity labeling tools and exploring their application in elucidating the spatiotemporal regulation of RNA at the subcellular level.

关 键 词：RNA localization proximity labeling spatiotemporal regulation subcellular compartments 

分 类 号：R73[医药卫生—肿瘤]