The biogenesis,regulation and functions of transitive siRNA in plants  

作　　者：Huijun Tan Yuelin Liu Hongwei Guo 

机构地区：[1]Shenzhen Key Laboratory of Plant Genetic Engineering and Molecular Design,Institute of Plant and Food Science,Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China [2]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [3]Department of Biology,Hong Kong Baptist University,Hong Kong SAR,China

出　　处：《Acta Biochimica et Biophysica Sinica》2025年第1期131-147,共17页生物化学与生物物理学报(英文版)

基　　金：supported by the grants from the National Natural Science Foundation of China(No.32261160572 to H.G.);the New Cornerstone Science Foundation(No.NCI202235 to H.G);Shenzhen Science and Technology Program(Nos.KQTD20190929173906742 and ZDSYS20230626091659010 to H.G.,JCYJ20190809163019421 to Y.L.);Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture(to H.G.).

摘　　要：Small RNA(sRNA)-mediated RNA interference(RNAi)is a sequence-specific gene silencing mechanism that modulates gene expression in eukaryotes.As core molecules of RNAi,various sRNAs are encoded in the plant genome or derived from invading RNA molecules,and their biogenesis depends on distinct genetic pathways.Transitive small interfering RNAs(siRNAs),which are sRNAs produced from double-strand RNA(dsRNA)in a process that depends on RNA-dependent RNA polymerases(RDRs),can amplify and spread silencing signals to additional transcripts,thereby enabling a phenomenon termed“transitive RNAi”.Members of this class of siRNAs function in various biological processes ranging from development to stress adaptation.In Arabidopsis thaliana,two RDRs participate in the generation of transitive siRNAs,acting cooperatively with various siRNA generation-related factors,such as the RNA-induced silencing complex(RISC)and aberrant RNAs.Transitive siRNAs are produced in diverse subcellular locations and structures under the control of various mechanisms,highlighting the intricacies of their biogenesis and functions.In this review,we discuss recent advances in understanding the molecular events of transitive siRNA biogenesis and its regulation,with a particular focus on factors involved in RDR recruitment.We aim to provide a comprehensive description of the generalized mechanism governing the biogenesis of transitive siRNAs.Additionally,we present an overview of the diverse biological functions of these siRNAs and raise some pressing questions in this area for further investigation.

关 键 词：SILENCING SIRNA TRANSITIVITY RDRs 

分 类 号：O15[理学—数学]