猫泛白细胞减少症病毒VP2蛋白基因重组杆状病毒的构建及应用  

Expression of VP2 Protein of Feline Panleucopenia in Baculovirus System

作　　者：郭伟伟 向银辉 刘大卫 刘岳 孙鹏 崔晓霞 杜元钊 GUO Weiwei;XIANG Yinhui;LIU Dawei;LIU Yue;SUN Peng;CUI Xiaoxia;DU Yuanzhao(Qingdao Yebio Biological Engineering Co.Ltd,Qingdao 266000,China)

机构地区：[1]青岛易邦生物工程有限公司,青岛266000

出　　处：《中国动物传染病学报》2025年第1期91-96,共6页Chinese Journal of Animal Infectious Diseases

摘　　要：本研究将猫泛白细胞减少症病毒FPV-SD株VP2基因克隆后,构建重组Bacmid-VP2,转染昆虫细胞Sf9,获得了重组杆状病毒re-BacVP2,并对其进行一系列鉴定。结果显示:其病毒含量可达到107.1 TCID50/mL。SDS-PAGE、Western blot结果证明猫泛白细胞减少症病毒VP2基因在杆状病毒表达系统中成功表达,并与抗FPV的多克隆抗体发生特异性反应。该蛋白能凝集1%猪红细胞,凝集价达1∶16384。电镜观察可见表达的VP2蛋白能形成病毒样颗粒(VLPs),为20~22 nm。VP2蛋白制备的疫苗能产生较高的抗体滴度。VP2基因的成功表达为FPV疫苗的研制奠定基础。The VP2 gene of Feline parvovirus(FPV)SD strain was amplified to construct a recombinant Bacmid.Recombinant baculovirus re-BacVP2 was obtained by transfecting Bacmid-vp2 into insect cell SF9 and the expressed VP2 protein was identified in SDS-PAGE analysis Its reactivity was demonstrated in Western blot.The VP2 protein agglutinated 1%of porcine erythrocytes,and HA titer was 14 log2.In addition,the VP2 protein formed virus-like particles(VLPs)with the size of 20-22 nm.The VP2 vaccine induced high antibody titers.Expression of the VP2 protein in sf9 cells laid a solid foundation for development of FPV vaccines.

关 键 词：猫泛白细胞减少症病毒 VP2基因 重组杆状病毒 鉴定 

分 类 号：S859.797[农业科学—临床兽医学]