我国部分地区牛冠状病毒S基因遗传变异分析  

Genetic Variation Analysis of S Gene of Bovine Coronavirus in Some Regions of China

作　　者：张欣怡 高辉 崔进 房琳琳 郑辉[2] 刘爽[2] 魏荣[2] 李晓成[2] 肖啸[1] 董雅琴[2,3] ZHANG Xinyi;GAO Hui;CUI Jin;FANG Linlin;ZHENG Hui;LIU Shuang;WEI Rong;LI Xiaocheng;XIAO Xiao;DONG Yaqin(Yunnan Agricultural University,Kunming 650500,China;China Animal Health and Epidemiology Center,Qingdao 266032,China;Key Laboratory of Animal Biosafety Risk Prevention and Control(South),Qingdao 266032,China;Zhaotong Vocational Education Center,Zhaotong 657000,China)

机构地区：[1]云南农业大学,昆明650500 [2]中国动物卫生与流行病学中心,青岛266032 [3]农业农村部动物生物安全风险预警及防控重点实验室(南方),青岛266032 [4]昭通市职业教育中心,昭通657000

出　　处：《中国动物传染病学报》2025年第1期188-198,共11页Chinese Journal of Animal Infectious Diseases

基　　金：农业农村部动物冠状病毒监测预警项目(16220223)。

摘　　要：为了解我国部分地区牛冠状病毒(BCoV)流行及变异情况,2020—2021年,收集我国9个省份53个临床发病牛群的拭子、粪便、组织等样品1432份,采用实时荧光RT-PCR方法进行BCoV检测,并选取部分核酸阳性样品进行S基因全长的扩增、测序与遗传变异分析。53个发病牛群中,BCoV阳性场占54.72%,冬季BCoV感染率(14.87%)最高,犊牛最易感(16.13%)。本研究共获得17条S基因全长序列与参考序列核苷酸同源性为96.7%~99.7%。系统进化分析表明,BCoV毒株分为欧洲(GⅠ型)和亚-美(GⅡ型)两大分支,所测毒株均属GⅡ型,且与大多中国参考毒株共同位于GⅡ中相对独立的分支。与中国参考毒株相比,所测毒株存在60个氨基酸变异位点,所测4个内蒙古毒株存在16个有别于其他测序株的相同突变,推测与中国参考株HLJ/HH-10/2020、BCoV-GX-NN190313有相同的祖先。该研究为揭示我国BCoV流行情况及流行毒株分子特征提供参考。In order to investigate the prevalence and genetic variation of Bovine coronavirus(BCoV)in parts of China,a total of 1432 swab,fecal,blood and tissue samples were collected from 2020 to 2021 from 53 cattle herds with clinical signs from 9 provinces of China.Realtime fl uorescence RT-PCR was used to test BCoV nucleic acids in these samples and the full-length of S gene of the selected positive samples was amplified for sequence analysis.The positive rate was 54.72%in 53 herds,among which the highest prevalence occurred in winter(14.87%)and calf(16.13%)seemed to be most susceptible.In our study,the full-length sequences of S gene were obtained from 17 strains and the nucleotide homology between them and the reference strains was 96.7%-99.7%.Phylogenetic analysis showed BCoV strains were divided into two branches genetically,i.e.the European branch(type GⅠ)and Asia-American branch(type GⅡ).All of these 17 strains belonged to GⅡwith most of Chinese reference strains.Compared to the Chinese reference strains,there were mutations at 60 amino acid residues in these 17 strains.Additionally,4 strains from Inner Mongolia possessed 16 distinct mutations,which was speculated that these 4 strains shared the same ancestors with the Chinese reference strains HLJ/HH-10/2020 and BCoV-GX-NN190313.In summary,our study provided scientific support for the prevalence and molecular features of BCoV in China.

关 键 词：牛冠状病毒 S基因 系统进化分析 氨基酸变异 

分 类 号：S852.65[农业科学—基础兽医学]