苏北鲁南地区PRRSV流行病学调查  

Epidemiological Investigation of PRRSV in Northern Jiangsu and Southern Shandong

作　　者：周庆雨 张巍巍 买尔外提·波拉提 卢玮 沙黑拉·巴合提 罗林 包文武 张秀玉 ZHOU Qingyu;ZHANG Weiwei;MAIERWAITI·Bolati;LU Wei;SHAHEILA·Baheti;LUO Lin;BAO Wenwu;ZHANG Xiuyu(Lianyungang Chia Tai Agro-Industry Development Co.,LTD,Lianyungang 222000,China)

机构地区：[1]连云港正大农牧发展有限公司动保中心实验室,连云港222000

出　　处：《中国动物传染病学报》2025年第1期199-205,共7页Chinese Journal of Animal Infectious Diseases

摘　　要：本调查对苏北鲁南地区30余个规模化养殖场送检的2894份样品进行RT-PCR检测,通过检测数据统计分析,确定了猪繁殖与呼吸综合征病毒(PRRSV)在本地区的流行趋势。同时选用NSP2基因序列特异性引物探针试剂盒,对300份阳性样本进行毒株鉴别,发现PRRSV高致病性变异株阳性率逐年攀升。进一步对Ct值较低的50份阳性样本进行NSP2、ORF5基因测序分析,锁定了苏北鲁南地区主要的PRRSV流行毒株。In this study,2894 samples from more than 30 large-scale farms in northern Jiangsu and southern Shandong were detected by RT-PCR.The NSP2 gene sequence-specific primer probe kit was used to identify the strains of 300 positive samples.It was found that the positive rate of highly pathogenic PRRSV mutants was increasing year by year.Further,50 positive samples with low Ct values were sequenced and analyzed for NSP2 and ORF5 genes,and the main PRRSV epidemic strains in northern Jiangsu and southern Shandong were identified.

关 键 词：PRRSV 类NADC30 PRRSV高致病性变异株 基因测序 

分 类 号：S852.618[农业科学—基础兽医学]