电针抑制PI3K/Akt/NF-κB通路激活缓解大鼠子宫内膜异位症  

Electroacupuncture Inhibits PI3K/Akt/NF-κB Pathway Activation to Alleviate Endometriosis in Rats

作　　者：杨阳 田丹丹 盛刚 YANG Yang;TIAN Dandan(Northwest Women and Children's Hospital,Shaanxi Xi'an 710061,China)

机构地区：[1]西北妇女儿童医院中医科,陕西西安710061 [2]陕西省中医医院针灸科,陕西西安710010

出　　处：《河北医学》2025年第3期386-390,共5页Hebei Medicine

基　　金：陕西省重点研发计划项目,(编号:2021SF-2201)。

摘　　要：目的:探讨电针对大鼠子宫内膜异位症(EMS)作用以及对PI3K/Akt/NF-κB通路的影响。方法:构建EMS大鼠模型,随机分为假手术组(n=20)、模型组(n=20)、电针组(n=20)治疗28d后分析异位子宫内膜体积,苏木精-伊红染色分析子宫内膜病理形态学变化。采用Western blot和酶联免疫吸附试验评估PI3K/Akt/NF-κB通路中的蛋白质表达和细胞因子水平。结果:与假手术组相比,模型组大鼠异位子宫内膜的质量和体积明显增加(P<0.05)。与模型大鼠相比,电针组的大鼠异位子宫内膜的质量和体积明显减少(P<0.05)。在组织形态学上,电针治疗也明显减轻了子宫内膜腺体的增生和空泡化、炎性细胞浸润和腺体与基质比例的增加以及间质病变的增厚。其次,与假手术组相比,模型组大鼠血清和腹膜液中TNF-α和IL-6的水平显著提高(P<0.05);而电针治疗可以降低TNF-α和IL-6的水平(P<0.05)。此外,与假手术组相比,模型组p-PI3K、p-AKT和p-p65蛋白表达显著提高;而与模型组相比,电针组p-PI3K、p-AKT和p-p65蛋白表达显著降低。结论:电针通过抑制PI3K/Akt通路和NF-κB通路的活性,从而防止大鼠EMS的发生。Objective:To investigate the effects of electroacupuncture(EA)on endometriosis(EMS)in rats and its influence on the PI3K/Akt/NF-κB pathway.Methods:An EMS rat model was established and randomly divided into a sham surgery group(n=20),a model group(n=20),and an electroacupuncture group(n=20).After 28 days of treatment,the volume of ectopic endometrium was analyzed,and hematoxylin-eosin(H&E)staining was used to assess pathological morphological changes in the endometrium.Western blot and enzyme-linked immunosorbent assay(ELISA)were employed to evaluate protein expression and cytokine levels in the PI3K/Akt/NF-κB pathway.Results:Compared with the sham surgery group,the mass and volume of ectopic endometrium in the model group were significantly increased(P<0.05).In contrast,the electroacupuncture group showed a significant reduction in the mass and volume of ectopic endometrium compared to the model group(P<0.05).Histomorphologically,electroacupuncture treatment significantly alleviated endometrial gland hyperplasia,vacuolization,inflammatory cell infiltration,increased gland-to-stroma ratio,and stromal thickening.Furthermore,compared to the sham surgery group,the levels of TNF-αand IL-6 in the serum and peritoneal fluid of the model group were significantly elevated(P<0.05),while electroacupuncture treatment reduced the levels of TNF-αand IL-6(P<0.05).Additionally,the expression of p-PI3K,p-AKT,and p-p65 proteins was significantly increased in the model group compared to the sham surgery group,whereas electroacupuncture treatment significantly decreased the expression of these proteins compared to the model group.Conclusion:Electroacupuncture prevents the occurrence of EMS in rats by inhibiting the activity of the PI3K/Akt and NF-κB pathways.

关 键 词：电针 PI3K/Akt/NF-κB通路 子宫内膜异位症 

分 类 号：R73[医药卫生—肿瘤]