硬枝黄蝉中4种环烯醚萜类化合物对辣椒疫霉菌的抑菌活性研究  

Study on the Antifungal Activity of Four Iridoids from Allemanda neriifolia Against Phytophthora capsici

作　　者：苟亚峰[1,2] 田甜 温思为 刘世超 高圣风 孙世伟[1] GOU Yafeng;TIAN Tian;WEN Siwei;LIU Shichao;GAO Shengfeng;SUN Shiwei(Research Institute of Spice-and-Beverage Crops,Chinese Academy of Tropical Agricultural Sciences,Wanning,Hainan 571533,China;Key Laboratory of Processing-Suitability and Quality Control of the Special Tropical Crops of Hainan Province,Wanning,Hainan 571533,China)

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]海南省特色热带作物适宜性加工与品质控制重点实验室,海南万宁571533

出　　处：《热带农业科学》2025年第2期33-36,共4页Chinese Journal of Tropical Agriculture

基　　金：海南省自然科学基金项目“硬枝黄蝉中环烯醚萜类化合物对辣椒疫霉菌的抑菌机理研究”(No.321MS092);海南省自然科学基金项目“诺丽提取物对咖啡炭疽病菌抑菌活性研究”(No.322QN401)。

摘　　要：环烯醚萜类化合物作为众多植物中的有效成分之一,具有广泛的生物活性,如抗病毒、抗菌、抗肿瘤、抗氧化等,前期从硬枝黄蝉中分离得到4种环烯醚萜类化合物:plumieride、protoplumericinA、13-dexoylplumieride和13-O-p-coumaroylplumieride。采用生长速率法测定了上述4种化合物对辣椒疫霉菌的抑菌活性,并通过扫描电镜观察抑菌活性化合物对辣椒疫霉菌表观形态的影响。结果表明,供试化合物中protoplumericinA和13-O-p-coumaroyl-plumieride对辣椒疫霉菌具有一定抑菌活性,抑制率分别达到了72.97%和69.92%;采用13-O-P-coumaroylplumieride和protoplumierideA处理后,菌丝出现不规则收缩,表面塌陷或模糊等症状。研究为进一步研制防治辣椒疫霉菌的植物源杀菌剂提供支撑。As components of many plants,iridoids have a wide range of biological activities,such as antiviral,antibacterial,anti-tumor,antioxidant,etc.Four iridoids of plumieride,protoplumericin A,13-dexoylplumieride and 13-O-p-coumaroylplumieride have been isolated from Allemanda neriifolia.The antifungal activity against Phytophthora capsici was measured via the growth rate method,and the changes in the morphology of P.capsici were observed by scanning electron microscopy.The results showed that protoplumericin A and 13-O-p-coumaroylplumieride had high antifungal activities,which inhibited fungal growth by 72.97%and 69.92%,respectively.Scanning electron microscope observation revealed irregular mycelial shrinkage,surface collapse,or fuzzy symptoms.This study provides support for the further development of plant-source fungicides to control P.capsici.

关 键 词：硬枝黄蝉 辣椒疫霉菌 环烯醚萜类 抑菌活性 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]