黄曲霉毒素B_(1)/呕吐毒素/玉米赤霉烯酮三合一时间分辨荧光定量快速检测卡的样品稀释液优化  

Optimization of Sample Diluent for Aflatoxin B_(1)/Vomitoxin/zearalenone Three-in-one Time-resolved Fluorescence Quantitative Rapid Detection Card

作　　者：陈家伟 谈婷 丁丽 王星 肖理文 CHEN Jiawei;TAN Ting;DING Li;WANG Xing;XIAO Liwen(Nanjing Microdetection Bio-Tech Co.,Ltd.,210031 Nanjing,China;China Grain Storage Anhui Quality Inspection Center Co.,Ltd.,230061 Hefei,China)

机构地区：[1]南京微测生物科技有限公司,南京210031 [2]中储粮安徽质检中心有限公司,合肥230061

出　　处：《粮食加工》2025年第2期114-119,131,共7页Grain Processing

基　　金：江苏省社会发展项目(BE2022688)。

摘　　要：在之前研究的基础上,对黄曲霉毒素B_(1)/呕吐毒素/玉米赤霉烯酮三合一(以下简称ADZ)时间分辨荧光定量快速检测卡适配的样品稀释液进行优化实验。兼顾标准品检测性能,结合真实样本,对ADZ荧光定量快速检测卡的样品稀释液pH、离子浓度、表面活性剂进行较为系统的实验验证。不仅提高了样本检测的均一性与准确度AFB_(1)(浓度CV:4%~10%,准确度:96%~110%),DON(浓度CV:2%~11%,准确度:92%~110%),ZEN(浓度CV:3%~12%,准确度:97%~109%)),也解决了部分饲料厂商DDGS样本检测时的乳胶残留问题,还消除了一些样本的假阳性问题(例如麦胚)。该研究极大提高了ADZ荧光定量快速检测卡的样本兼容性,为各大食品安全检测机构、粮油饲料厂商提供了更加可靠、更加高效的快检产品。This paper is based on the research of Aflatoxin B_(1)/vomitoxin/zearalenone three-in-one(ADZ)timeresolved fluorescence quantitative rapid detection card of Tan Ting et al.,and the optimization experiment of its suitable sample dilution was carried out.The pH,ion concentration and surfactant of the sample diluent of the ADZ fluorescence quantitative rapid detection card were verified systematically.It not only improves the uniformity and accuracy of sample detection(AFB_(1)(concentration CV:4%~10%,accuracy:96%~110%),DON(concentration CV:2%~11%,accuracy:92~110%),ZEN(concentration CV:3%~12%,accuracy:97%~109%),and also solved the latex residue problem in DDGS samples tested by some feed manufacturers,and also eliminated the false positive problem in some samples(for example,wheat germ).This study greatly improves the sample compatibility of our ADZ fluorescent quantitative rapid detection card,and provides a more reliable and efficient rapid detection of products for major food safety testing institutions and grain,oil and feed manufacturers.

关 键 词：真菌毒素多合一 快速定量检测 荧光定量免疫层析 样品稀释液 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]