An activated fluorescent probe to monitor NO fluctuation in Parkinson’s disease  

作　　者：Tao Liu Xuwei Han Xueyi Sun Weijie Zhang Ke Gao Runan Min Yuting Tian Caixia Yin 

机构地区：[1]Department of Chemical and Materials Engineering,Lvliang University,Lvliang 033001,China [2]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Molecular Science,Shanxi University,Taiyuan 030006,China [3]School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China

出　　处：《Chinese Chemical Letters》2025年第3期341-344,共4页中国化学快报(英文版)

基　　金：the Program of Graduate Education and Teaching Reform of Shanxi(No.2022YJJG302);the Applied Basic Research Programs of Shanxi(Nos.201801D221106,202203021221228);the Key R&D Project of Lvliang(No.2023GXYF04);Scientific Instrument Center of Shanxi University(No.201512)。

摘　　要：The imbalance of nitric oxide(NO)homeostasis in the brain is closely related to the occurrence of Parkinson’s disease(PD).Therefore,revealing the fluctuation of NO in brain is crucial for understanding the pathophysiological processes.However,currently developed NO probes are unsuitable for this purpose due to their poor blood-brain barrier permeability.Herein,a fluorescent probe(PO-NH)with blood-brain barrier crossing capability and high selectivity for NO was developed.Under the NO mediation,the photoinduced electron transfer(PET)process of the probe was blocked,giving an intensive fluorescence enhancement(F/F_(0)=15).Moreover,PO-NH can be used to effectively monitor changes in intracellular NO levels.Significantly,due to excellent blood-brain barrier crossing ability and near-infrared(NIR)emission,PO-NH is suitable for in vivo imaging of NO in the brain and illustrating with the deterioration of PD,the level of NO gradually increased in the brain of PD mice.We believe that PO-NH may provide a beneficial tool for understanding the biological function of NO in the brain and revealing the complex connection between NO and PD.

关 键 词：Blood brain barrier Nitric oxide Fluorescent probe Turn off/on Parkinson’s disease 

分 类 号：R742.5[医药卫生—神经病学与精神病学]