两株植物促生细菌的分离鉴定及对马铃薯的促生效果评价  

Isolation and Identification of Two Plant Growth-promoting Bacteria and Evaluation of Their Growth-promoting Effects on Potatoes

作　　者：孙平平 吴若晨 吕秀华 贾伟 张磊[1] 李正男 SUN Pingping;WU Ruochen;LYU Xiuhua;JIAWei;ZHANG Lei;LI Zhengnan(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018,China;Inner Mongolia Preschool Education College for Nationalities,Erdos,Inner Mongolia 017000,China)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特010018 [2]内蒙古民族幼儿师范高等专科学校,内蒙古鄂尔多斯017000

出　　处：《中国马铃薯》2024年第4期339-346,共8页Chinese Potato Journal

基　　金：鄂尔多斯市重点研发计划项目(YF20240042);内蒙古自治区科技计划项目(2021GG0112);内蒙古自治区高等学校科学技术研究项目(NJZY21458)。

摘　　要：根际促生菌的应用是农业“减肥、减药”的有效措施。为筛选更多根际促生菌资源,以陕西省韩城市铁矿厂土样为试验材料,采用逐级稀释法、促生筛选培养基和马铃薯盆栽试验筛选有促生效果的菌株,获得了两株植物根际促生菌,并研究其促生效果及对p H和盐的耐受性。从铁矿厂分离筛选到2株具有良好促生作用的菌株HC6-7和HC6-11,均具有络合铁和产IAA能力,其中菌株HC6-7可产生11.59 mg/L的IAA,菌株HC6-11具有固氮和解磷能力;2株菌最适生长pH为7~9,均可在浓度为5%NaCl及以下培养基中生长。马铃薯盆栽结果显示,经菌液处理后,处理组生物量均高于对照组;形态学和分子生物学方法鉴定菌株HC6-7为内生芽孢杆菌(Priestia endophytica),HC6-11为阿塔卡马假单胞菌(Pseudomonas atacamensis)。内生芽孢杆菌(P.endophytica HC6-7)和阿塔卡马假单胞菌(P.atacamensis HC6-11)对马铃薯具有促生能力,该结果将为植物促生菌肥研制提供微生物资源。The application of plant growth-promoting bacteria is an effective method for reducing the need for pesticides and fertilizers.To isolate additional resources of plant growth-promoting bacteria,soil samples were collected from the Hancheng Iron Ore site in Shaanxi Province for this research.The serial dilution method,in vitro cultivation on growth-promoting screening medium,and in vivo screening on potato plants were employed to select the growth-promoting strains.Two strains were identified,and their tolerance to pH and salt concentration was studied.The two strains,HC6-7 and HC6-11,exhibiting significant growth-promoting effects,were isolated from the iron mine.Both strains demonstrated the ability to produce siderophores and indole-3-acetic acid(IAA),with HC6-7 producing IAA at a concentration of 11.59 mg/L.Additionally,strain HC6-11 exhibited capabilities for nitrogen fixation and phosphorus solubilization.The optimal pH for both strains ranged from 7 to 9,and both strains were able to grow in media containing 5%or less NaCl.Pot experiments with potato plants revealed that the biomass of potatoes treated with the bacterial solution was greater than that of the control group.The strain HC6-7 was identified as Priestia endophytica,while HC6-11 was identified as Pseudomonas atacamensis through morphological and molecular biology methods.In conclusion,P.endophytica HC6-7 and P.atacamensis HC6-11 have the potential to promote potato growth,and these findings would provide valuable microbial resources for the development of plant growth-promoting bacterial fertilizer.

关 键 词：马铃薯 促生菌 内生芽孢杆菌 阿塔卡马假单胞菌 

分 类 号：S532[农业科学—作物学]