PAX1和SOX14甲基化检测对高危型人乳头瘤病毒感染进展为子宫颈鳞状上皮内病变的分流作用  

Investigating the triage effect of PAX1 and SOX14 methylation detection on the progression of high risk HPV infection into cervical squamous

作　　者：阮颖 罗帅 谭娜 王进京 郑洪 RUAN Ying;LUO Shuai;TAN Na;WANG Jin-jing;ZHENG Hong(Department of Pathology,Affiliated Hospital of Zunyi Medical University,Zunyi,Guizhou 563000,China)

机构地区：[1]遵义医科大学附属医院病理科,贵州遵义563000

出　　处：《中国妇幼保健》2025年第6期1098-1102,共5页Maternal and Child Health Care of China

基　　金：贵州省卫生健康委科学技术基金项目(gzwkj2022-192);贵州省遵义市科技计划项目[遵市科合HZ字(2021)120号]。

摘　　要：目的探讨PAX1、SOX14甲基化检测对高危型HPV感染进展为子宫颈鳞状上皮内病变的分流作用。方法收集2021年AptimaHPVE6/E7mRNA检测标本,分为HPV18/45+组、HPV16+组、其余型别HPV+组及HPV-对照组并随机抽样90例,采用焦磷酸测序法进行PAX1、SOX14甲基化位点定量检测,分析不同位点、不同分组之间差异是否具有统计学意义,随访HPVE6/E7mRNA阳性者活检病理结果判断甲基化检测诊断效能,并以病理结果整理甲基化检测值,分析不同病变级别病理结果之间差异是否具有统计学意义。结果PAX1甲基化检测位点比较,组间(1、3、5、8)位点有差异(P<0.05);SOX14甲基化检测位点比较,组间(2、4、5)位点差异有统计学意义(P<0.05)。4组PAX1、SOX14甲基化定量检测,组间平均值差异有统计学意义(P<0.0001),且HPV16+组与HPV-组、HPV18/45+组、其余+组差异均有统计学意义(P<0.0001)。随访活检病理结果72例,PAX1、SOX14甲基化单独与联合检测以HPV-组平均值作为对照,诊断CIN1+病变灵敏度、特异度、误诊率、漏诊率、Kappa值、ROC曲线下面积为(86.36%、92.86%、7.14、13.64%、0.772、0.896、77.27%、100%、0、22.72%、0.726、0.886、90.91%、92.86%、7.14%、9.09%、0.827、0.919)。不同病变级别病理结果组间PAX1、SOX14甲基化检测位点平均值差异有统计学意义(P<0.0001)。结论PAX1、SOX14甲基化定量检测在HPVE6/E7mRNA 16型具有较高的甲基化率。PAX1、SOX14甲基化定量联合检测对HPVE6/E7mRNA阳性进展为CIN1+具有更好的病理诊断一致性,有助于鳞状上皮内病变的分流。Objective To investigate the triage effect of PAX1 and SOX14 methylation detection on the progression of high risk HPV infection into cervical squamous intraepithelial lesions.Methods AptimaHPVE6/E7mRNA samples were collected and divided into HPV18/45+group in 2021,HPV 16+group,other types of HPV+group and HPV-control group,and 90 cases were randomly sampled.Pyrosequencing method was used for quantitative detection of PAX1 and SOX14 methylation sites.To analyze whether there were statistical differences between different sites and different groups,follow up the biopsy pathological results of HPVE6/E7mRNA infected persons to determine the diagnostic efficacy of methylation detection,and collate the methylation detection values based on the pathological results to analyze whether there were statistical differences between the pathological results of different lesion levels.Results PAX1 methylation detection sites were different among groups(1,3,5,8)(P<0.05).Comparison of SOX14 methylation detection sites showed differences among groups(2,4,5)(P<0.05).Quantitative methylation of PAX1 and SOX14 in the four groups showed statistically significant differences in mean values between the groups(P<0.0001),and statistically significant differences between HPV16+group and HPV-group,HPV18/45+group and other+groups(P<0.0001).Follow-up biopsy pathological results of 72 cases,PAX1,SOX14 methylation alone and combined detection using HPV-group mean value as control,Diagnosis of CIN1+lesion sensitivity,specificity,misdiagnosis rate,missed diagnosis rate,Kappa value,area under ROC curve was 86.36%,92.86%,7.14,13.64%,0.772,0.896,77.27%,100%,0,22.72%,0.726,0.886,90.91%,92.86%,7.14%,9.09%,0.827,0.919).The mean values of PAX1 and SOX14 methylation detection sites were statistically different among different pathological grades(P<0.0001).Conclusion PAX1,SOX14 methylation quantitative detection of HPVE6/E7mRNA type 16 has a higher methylation rate.The combined quantitative detection of PAX1 and SOX14 methylation has a better consistency

关 键 词：PAX1 SOX14 甲基化 HPVE6/E7mRNA 鳞状上皮内病变 

分 类 号：R737.33[医药卫生—肿瘤]