猕猴桃软腐病菌早期侵染过程的转录组学分析  

Transcriptomic Analysis of Botryosphaeria dothidea on Kiwifruit during the Early Infection Process

作　　者：宋金黄 谭红 赵志博 龙友华[1] 胡小平[2] 樊荣 SONG Jinhuang;TAN Hong;ZHAO Zhibo;LONG Youhua;HU Xiaoping;FAN Rong(College of Agriculture,Guizhou University,Guiyang 550025,China;State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,Yangling 712100,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《食品科学》2025年第8期92-100,共9页Food Science

基　　金：国家自然科学基金地区科学基金项目(32260658);国家自然科学基金青年科学基金项目(31901836);贵州省自然科学基金项目(黔科合基础-ZK[2022]一般048)。

摘　　要：基于RNA-Seq测序技术分析葡萄座腔菌(Botryosphaeria dothidea)在猕猴桃果实初侵染过程中的差异基因,旨在从转录调控水平上明确猕猴桃软腐病的发生机制,以期为该病害的科学防控提供新线索。结果表明,在侵染24 h和48 h分别有上调差异基因658个和921个,下调差异基因1951个和1979个;差异基因显著富集在病菌的核糖体、淀粉和蔗糖代谢、氨基糖和核苷酸糖代谢等途径中,参与碳源利用、有性繁殖和毒力等信号通路;将接种24 h和48 h持续上调表达的48个关键基因构建相关性网络,明确了处于网络节点的重要差异基因。本研究可为筛选猕猴桃软腐病菌重要致病基因提供科学依据。In this study,RNA sequencing(RNA-Seq)was employed to analyze differential gene expression during the initial stage of kiwifruit infection by Botryosphaeria dothidea,aiming to clarify the mechanism of kiwifruit soft rot at the level of transcriptional regulation in order to provide new clues for the scientific prevention and control of this disease.Results showed that 658 and 921 up-regulated genes,and 1951 and 1979 down-regulated genes were identified at 24 h and 48 h post inoculation,respectively.Differentially expressed genes(DEGs)were significantly enriched in pathways related to ribosomes,starch and sucrose metabolism,amino sugar and nucleotide sugar metabolism and also involved in carbon source utilization,sexual reproduction,virulence,and other signaling pathways.A correlation network was constructed of 48 key genes that were up-regulated at both 24 h and 48 h post inoculation,and the key DEGs at the network nodes were identified.This study provides a scientific basis for screening important pathogenic genes of kiwifruit soft rot disease.

关 键 词：猕猴桃 软腐病 葡萄座腔菌 侵染机制 致病基因 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]