桔梗皂苷G1通过影响细胞自噬抑制肝细胞衰老  

Platycoside G1 Inhibits Hepatocyte Senescence by Regulating Autophagy

作　　者：李一权 李雅茹 刘芸芸 赵仁双 赵哲皞 吴长征 张彪 LI Yiquan;LI Yaru;LIU Yunyun;ZHAO Renshuang;ZHAO Zhehao;WU Changzheng;ZHANG Biao(Academician Workstation,Changchun University of Chinese Medicine,Changchun 130117,China;Medical College,Yanbian University,Yanji 133002,China;College of Animal Sciences,Zhejiang University,Hangzhou 310013,China;School of Management,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学院士工作室,吉林长春130117 [2]延边大学医学院,吉林延吉133002 [3]浙江大学动物科学学院,浙江杭州310013 [4]长春中医药大学管理学院,吉林长春130117

出　　处：《食品科学》2025年第8期162-169,共8页Food Science

基　　金：长春中医药大学青年优秀学科骨干培养项目(202318)。

摘　　要：为研究桔梗皂苷G1(platycoside G1,PG1)对衰老细胞的影响,首先通过β-半乳糖苷酶染色和CCK-8检测确定PG1对衰老表型肝细胞有特异性抑制作用。随后使用流式分析、免疫荧光和Western blot法进一步证明了PG1主要通过自噬作用抑制衰老细胞。此外,利用XFe96代谢通量分析表明,PG1能显著诱导有氧糖酵解,并显著抑制线粒体代谢。总之,上述结果表明PG1对衰老细胞具有特异性抑制作用,本研究为进一步研究PG1的抗衰作用提供实验和理论依据。To investigate the effects of platycoside G1(PG1)on senescent cells,it was determined that PG1 exerts a specific inhibitory effect on the senescent phenotype of hepatocytes by employingβ-galactosidase staining and the cell counting kit-8(CCK-8)assay.Flow cytometry,immunofluorescence,and Western blot indicated that PG1 inhibited cellular senescence primarily through its regulatory effects on autophagy.Furthermore,XFe96 metabolic flux analysis revealed that PG1 significantly enhanced aerobic glycolysis while concurrently inhibiting mitochondrial metabolism.In conclusion,PG1 specifically inhibits cellular senescence.This study provides both experimental and theoretical foundations for the development of PG1-based anti-aging therapeutics.

关 键 词：细胞衰老 抗衰老 特异性 桔梗皂苷G1 自噬 

分 类 号：R966[医药卫生—药理学]