茶树泛素连接酶基因CsPUB21对非生物胁迫的响应  

Response of Ubiquitin-ligase Gene CsPUB21 to Different Abiotic Stress in Camellia sinensis

作　　者：邓淑琴 高莹瑞 李雨桐 王瑛[1] 龚春梅 白娟 DENG Shuqin;GAO Yingrui;LI Yutong;WANG Ying;GONG Chunmei;BAI Juan(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《园艺学报》2025年第3期655-670,共16页Acta Horticulturae Sinica

基　　金：陕西省重点研发计划项目(2022NY-167);西藏自治区科技计划项目(CGZH2024000047)。

摘　　要：以‘陕茶1号’茶树品种为材料,克隆得到U-box家族泛素E3连接酶基因CsPUB21并进行功能分析。序列分析发现CsPUB21编码区长度为1242 bp,共编码413个氨基酸,在N端含有U-box保守结构域、C端含有ARM结构域,为不稳定的亲水性蛋白。启动子区含有多个响应干旱、高盐、低温胁迫的顺式作用元件。亚细胞定位结果表明,CsPUB21定位于细胞膜和细胞质。基因表达模式分析显示,在5个供试茶树品种中,CsPUB21受干旱和盐胁迫诱导表达上调,而在低温胁迫下降低。在拟南芥中过表达CsPUB21,显著增强了植株对干旱和盐胁迫的抗性。qRT-PCR结果显示,CsPUB21过表达拟南芥在干旱和盐胁迫后CsPUB21表达量上调。与野生型相比,CsPUB21过表达拟南芥在干旱、盐胁迫处理条件下叶绿素含量、可溶性糖含量较高,抗氧化酶SOD、POD、CAT活性增强,相对电导率和MDA含量降低。研究结果说明CsPUB21在茶树应对干旱和盐胁迫时发挥正向调节作用。The U-box ubiquitin E3 ligase gene CsPUB21 were cloned and functionally analyzed in Camellia sinensis‘Shaancha 1’.The length of the coding sequence of CsPUB21 is 1242 bp,encoding a total of 413 amino acids with a conserved U-box domain at the N terminus and an ARM domain at the C terminus.The encoded protein,an unstable hydrophilic protein.The promoter region contains multiple cis-acting elements in response to drought,high salt and low temperature.The subcellular localization results indicated that CsPUB21 is localized on the cell membrane and cytoplasm.Gene expression pattern analysis showed that CsPUB21 was up-regulated by drought and salt stress treatments,and down-regulated by low temperature in different cultivars of C.sinensis.Overexpression of CsPUB21 gene in Arabidopsis significantly enhanced plants resistance to drought and salt stress.The qRT-PCR results showed that the expression level of CsPUB21 was up-regulated after drought and salt stress.Compared with the wild type,overexpression of CsPUB21 in Arabidopsis resulted in higher chlorophyll content and soluble sugar content under drought and salt stress,enhanced activities of antioxidant enzymes SOD,POD,and CAT,and decreased relative conductivity and MDA content.The research results indicated that the CsPUB21 plays a positive regulatory role in tea plant response to drought and salt stress.

关 键 词：茶树 CsPUB21 基因克隆 非生物胁迫 表达分析 异源功能验证 

分 类 号：S571.1[农业科学—茶叶生产加工]