The Effect of Serum Deprivation Response-related Protein on Hepatocellular Carcinoma Cells via the ASK1-JNK/p38 MAPK Signaling Pathway  

作　　者：LIN Yan-hui 

机构地区：[1]Fujian Provincial Hospital,Fuzhou Fujian 350001,China

出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》2024年第4期139-145,共7页中国生物医学工程学报(英文版)

基　　金：Fujian Provincial Health and Wellness Scientific Research Talent Development Program;grant number:2019-1-5

摘　　要：Objective:To investigate the effect of serum deprivation response-related protein(SDPR)on hepatocellular carcinoma(HCC)cells through the ASK1-JNK/p38 MAPK signaling pathway.Methods:A total of 30 HCC tissue samples and adjacent non-tumor tissues were collected from HCC patients who underwent hepatectomy in our hospital from June 2023 and June 2024.Quantitative real-time PCR and Western blot were used to measure the expression levels of SDPR,ASK1,JNK,and p38 MAPK in HCC tissues.Cell proliferation,migration,and apoptosis were compared between SDPR-low and SDPR-high HCC tissues.Results:SDPR m RNA levels and SDPR/GAPDH ratios in HCC tissues were significantly lower than those in adjacent tissues(P<0.05).The OD values of SDPR-low HCC tissues on Days 3,4,and 5 were significantly higher than those of SDPR-high HCC tissues(P<0.05).The number of migrated cells at 20 and 40 h in SDPR-low HCC tissues was significantly greater than in SDPR-high tissues,while the apoptosis rate was significantly lower(P<0.05).SDPR-low HCC tissues exhibited significantly reduced m RNA and protein expression levels of ASK1,JNK,and p38 MAPK compared to SDPR-high tissues(P<0.05).Conclusion:SDPR is downregulated in HCC tissues.Low SDPR expression promotes HCC cell proliferation and migration while inhibiting apoptosis,potentially through modulating the expression of proteins in the ASK1-JNK/p38 MAPK signaling pathway.

关 键 词：hepatocellular carcinoma serum deprivation response-related protein(SDPR) ASK1-JNK/p38 MAPK signaling pathway 

分 类 号：R735.7[医药卫生—肿瘤]