小干扰RNA干扰迁移诱导基因7对肝癌细胞HepG2增殖和迁移的影响  

Effects of small interfering RNA targeting migration-inducing gene 7 on the proliferation and migration of hepatocellular carcinoma cell line HepG2

作　　者：栾奕 李志艳 LUAN Yi;LI Zhiyan(Department of Radiology,Children’s Hospital of Nanjing Medical University,Nanjing 210000,Jiangsu,China;Department of Ultrasound,Shenzhen Third People’s Hospital,Shenzhen 518000,Guangdong,China)

机构地区：[1]南京医科大学附属儿童医院放射科,南京210000 [2]深圳市第三人民医院超声科,广东深圳518000

出　　处：《癌症进展》2025年第4期402-405,共4页Oncology Progress

基　　金：国家自然科学基金(82172563)。

摘　　要：目的通过小干扰RNA(siRNA)抑制迁移诱导基因7(MIG7)的表达,探讨其对肝癌细胞HepG2增殖和迁移的影响。方法通过RNA干扰技术敲低MIG7高表达细胞系中MIG7的表达。以siRNA敲除MIG7表达的HepG2细胞为实验组,未转染的HepG2细胞为对照组。采用四甲基偶氮唑蓝(MTT)法和流式细胞术检测HepG2细胞增殖情况与凋亡情况,采用细胞划痕实验检测HepG2细胞迁移情况。结果成功构建了siRNAMIG7序列。第7天,siRNA-MIG7组在MIG7被siRNA敲除后,肝癌细胞的增殖水平低于对照组,差异有统计学意义(P﹤0.05)。siRNA-MIG7组细胞凋亡率为(21.7±3.7)%,明显高于对照组的(10.0±1.5)%,差异有统计学意义(P﹤0.01)。敲除MIG7后24 h,siRNA-MIG7组肝癌细胞的迁移率明显低于对照组(P﹤0.01)。结论使用SiRNA-MIG7干扰肝癌细胞中MIG7的表达能有效抑制肝癌细胞增殖、迁移,促进细胞凋亡。Objective To investigate the effects of silencing migration-inducing gene 7(MIG7)expression by small interfering RNA(siRNA)on the proliferation and migration of hepatocellular carcinoma cell line HepG2.Method The expression of MIG7 in a high-expressing cell line was knocked down using RNA interference technology.HepG2 cells with MIG7 expression knocked out by siRNA were used as the experimental group,while untransfected HepG2 cells served as the control group.The proliferation and apoptosis of HepG2 cells were detected by methyl thiazolyl terazolium(MTT)assay and flow cytometry,respectively.The migration of HepG2 cells was detected by scratch assay.Result The siRNA-MIG7 sequence was successfully constructed.On the 7th day,the proliferation level of hepatocellular carcinoma cells in the siRNA-MIG7 group was significantly lower than that in the control group after MIG7 was knocked down by siRNA(P<0.05).The apoptosis rate of the siRNA-MIG7 group was(21.7±3.7)%,which was significantly higher than(10.0±1.5)%of the control group(P<0.01).After 24 h of MIG7 knockdown,the migration rate of hepatocellular carcinoma cells in the siRNA-MIG7 group was significantly lower than that in the control group(P<0.01).Conclusion Silencing MIG7 expression in hepatocellular carcinoma cells by siRNA-MIG7 can effectively inhibit cell proliferation and migration and promote apoptosis.

关 键 词：肝癌 迁移诱导基因7 小干扰RNA 

分 类 号：R735.7[医药卫生—肿瘤]