Dimensionality engineering of flower-like bimetallic nanozyme with high peroxidase-activity for naked-eye and on-site detection of acrylamide in thermally processed foods  

作　　者：Sen Chen Feifan Liu Taimei Cai Rong Wang Fangjian Ning Hailong Peng 

机构地区：[1]School of Chemistry and Chemical Engineering,Nanchang University,Nanchang,330031,China [2]School of Life Science,Jiangxi Science and Technology Normal University,Nanchang,330013,China [3]China Food Flavor and Nutrition Health Innovation Center,Beijing Technology and Business University,Beijing,100048,China

出　　处：《Nano Materials Science》2025年第1期123-133,共11页纳米材料科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(32060577 and 32360619);Natural Science Foundation of Jiangxi Province(20224ACB203016 and 20212BAB203034);the Open Project of China Food Flavor and Nutrition Health Innovation Center(CFC2023B-013).

摘　　要：Acrylamide(AA)is a neurotoxin and carcinogen that formed during the thermal food processing.Conventional quantification techniques are difficult to realize on-site detection of AA.Herein,a flower-like bimetallic FeCu nanozyme(FeCuzyme)sensor and portable platform were developed for naked-eye and on-site detection of AA.The FeCuzyme was successfully prepared and exhibited flower-like structure with 3D catalytic centers.Fe/Cu atoms were considered as active center and ligand frameworks were used as cofactor,resulting in collaborative substrate-binding features and remarkably peroxidase-like activity.During the catalytic process,the 3,3′,5,5′-tetrame-thylbenzidine(TMB)oxidation can be quenched by glutathione(GSH),and then restored after thiolene Michael addition reaction between GSH and AA.Given the“on–off–on”effect for TMB oxidation and high PODlike activity,FeCuzyme sensor exhibited a wide linear relationship from 0.50 to 18.00μM(R^(2)=0.9987)and high sensitivity(LOD=0.2360μM)with high stability.The practical application of FeCuzyme sensor was successfully validated by HPLC method.Furthermore,a FeCuzyme portable platform was designed with smartphone/laptop,and which can be used for naked-eye and on-site quantitative determination of AA in real food samples.This research provides a way for rational design of a novel nanozyme-based sensing platform for AA detection.

关 键 词：ACRYLAMIDE Glutathione Bimetallic FeCu nanozyme On-site detection Thermally processed food 

分 类 号：TS201.2[轻工技术与工程—食品科学]