长链非编码RNA HOTAIRM1对MPP^(+)处理后MN9D细胞活力和凋亡的影响  

Expression of the long non-coding RNA HOTAIRM1 on viability and apoptosis of MPP+-induced MN9D cells

作　　者：杨敏丽 张艺 高含 谭启涛 郑展越 孙天奥 潘明炼 马勇杰 孙艳 YANG Minli;ZHANG Yi;GAO Han;TAN Qitao;ZHENG Zhanyue;SUN Tianao;PAN Minglian;MA Yongjie;SUN Yan(School of Public Health,Guilin Medical University,Guilin 541199,Guangxi,China)

机构地区：[1]桂林医学院公共卫生学院,广西桂林541199

出　　处：《癌变.畸变.突变》2025年第2期128-133,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广西自然科学基金(2023GXNSFAA026035)。

摘　　要：目的:探讨长链非编码RNA(lncRNA)骨髓特异性HOX基因转录本反义RNA 1(HOTAIRM1)对1-甲基-4-苯基吡啶离子(MPP+)处理后的MN9D细胞活力和凋亡的影响。方法:将不同浓度(0、0.25、0.5、1、1.25、2.5 mmol/L)的MPP+作用于MN9D细胞不同时间(0、24、48和72 h)后,采用CCK-8检测各组细胞活力,选择MPP+最适浓度和最佳作用时间进行后续试验。选用siRNA进行转染,将MN9D细胞分为对照组、MPP+处理组(1 mmol/L MPP+处理24 h)、siRNA对照组(siRNA阴性对照序列转染1 h后用1 mmol/L MPP+处理24 h),和siR-HOTAIRM1干扰组(siR-HOTAIRM1转染1 h后用1 mmol/L MPP+处理24 h)。分别采用CCK-8检测各组细胞活力;实时荧光定量逆转录PCR(qRT-PCR)检测各组细胞中lncRNA HOTAIRM1的表达;流式细胞术检测各组细胞凋亡情况。结果:与对照组比较,不同浓度MPP+处理24 h,细胞活力随浓度的升高而降低(P<0.05),而lncRNA HOTAIRM1表达水平逐渐升高(P<0.05);1 mmol/L MPP+处理不同时间后,细胞活力明显下降(P<0.05),lncRNA HOTAIRM1表达水平先升高后降低(P<0.05),故选择1 mmol/L MPP+处理24 h进行后续试验。与转染siRNA阴性对照序列组相比,siR-HOTAIRM1干扰之后,lncRNA HOTAIRM1表达显著降低(P<0.01),但显著增强MPP+处理后的MN9D细胞的活力(P<0.01);明显抑制MPP+处理后的MN9D细胞的凋亡(P<0.01),从而保护MN9D细胞。结论:干扰lncRNA HOTAIRM1的表达可增强MPP+处理后的MN9D细胞活力,并抑制细胞凋亡。lncRNA HOTAIRM1敲低对MPP+处理后的MN9D细胞损伤具有保护作用。OBJECTIVE:To explore the impacts of the long non-coding RNA(lncRNA)HOX transcript antisense RNA,myeloid-specific 1(HOTAIRM1),on the vitality and apoptosis of MN9D cells which were induced by 1-methyl-4-phenylpyridinium ion(MPP+).METHODS:MN9D cells were treated with different concentrations(0,0.25,0.5,1,1.25,2.5 mmol/L)of MPP+for various time periods(0,24,48,and 72 h).Subsequently,cell viability of each group was evaluated using the CCK-8 assay.Based on the results,the optimal concentration of MPP+and the most appropriate treatment duration were determined for subsequent experiments.siRNA transfections were conducted.The MN9D cells were then categorized into the control group,MPP+group,si-NC+MPP+group,and si-HOTAIRM1+MPP+group.Cell viability of each group was measured by CCK-8 assay.Expression of lncRNA HOTAIRM1 was detected through real-time fluorescent quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Apoptosis status was analyzed by flow cytometry.RESULTS:In comparison with the control group,lncRNA HOTAIRM1 was highly expressed in MN9D cells induced by MPP+(P<0.05).When treated with 1 mmol/L MPP+for 24 h,the cell viability was significantly decreased,while the expression level of lncRNA HOTAIRM1 was remarkably elevated.Hence,1 mmol/L MPP+for 24 h treatment was selected for subsequent experiments.After the induction and siRNA interference,expression of lncRNA HOTAIRM1 was significantly reduced(P<0.001),and viability of the cells were significantly enhanced(P<0.01).Knockdown of lncRNA HOTAIRM1 inhibited the apoptosis of the induced cells(P<0.01).CONCLUSION:Interfering with the expression of lncRNA HOTAIRM1 augmented viability of the MN9D cells induced by MPP+and suppressed cell apoptosis.Knockdown of lncRNA HOTAIRM1 exerted a protective effect on the induced cells.

关 键 词：MN9D细胞 帕金森病 长链非编码RNA HOTAIRM1 细胞凋亡 

分 类 号：R741[医药卫生—神经病学与精神病学]