砂质土壤细菌群落对纳米氧化锌胁迫的响应机制研究  

Response mechanism of bacterial community to nano-zinc oxide stress in sandy soil

作　　者：彭东宇 林山杉[1] PENG Dongyu;LIN Shanshan(School of Environment,Northeast Normal University,Changchun 130117,China)

机构地区：[1]东北师范大学环境学院,吉林长春130117

出　　处：《东北师大学报(自然科学版)》2025年第1期144-153,共10页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金面上项目(41772236)。

摘　　要：采用室内土柱模拟实验,研究了纳米氧化锌(ZnO NPs)暴露下砂质土壤细菌群落的变化情况.结果表明:(1)ZnO NPs可显著降低土壤细菌的α多样性.短期实验土壤细菌群落间差异性较大,而长期实验其相似性较高.(2)Proteobacter、Arthrobacter分别为实验中绝对的优势菌门、属.短期实验ZnO NPs对Proteobacter门丰度的影响为高浓度促进、低浓度抑制,长期实验ZnO NPs对Proteobacter门丰度的影响与短期实验相反.短期实验ZnO NPs对Arthrobacter属丰度的影响为高浓度抑制、低浓度促进,长期实验ZnO NPs对Arthrobacter属丰度的影响与短期实验一致.(3)主要富集的细菌功能为氨基酸的运输和代谢.ZnO NPs显著影响了土壤细菌的转录、细胞内运输、分泌,囊泡运输等功能.To investigate the changes of bacterial communities in sandy soil under the exposure of zinc oxide nanoparticles(ZnO NPs),an indoor soil column simulation experiment was conducted.The results showed that:(1)ZnO NPs significantly reduced theα-diversity of soil bacteria.The differences among soil bacterial communities in short-term experiments were large,while the similarities among soil bacterial communities in long-term experiments were high.(2)Proteobacter and Arthrobacter were the absolute dominant phylum and genus in this experiment,respectively.In the short-term experiment,the effect of ZnO NPs on Proteobacter phylum abundance was promoted by high concentration and inhibited by low concentration.The effect of ZnO NPs on Proteobacter phylum abundance in the long-term experiment was opposite to that in the short-term experiment.The effect of ZnO NPs on Arthrobacter genus abundance in short-term experiments was inhibited by high concentration and promoted by low concentration.The effect of ZnO NPs on Arthrobacter genus abundance in the long-term experiment was consistent with that in the short-term experiment.(3)Amino acid transport and metabolism were the most abundant functions of all bacteria.ZnO NPs significantly affected transcription,intracellular transport,secretion,vesicle transport and other functions.

关 键 词：砂质土壤 纳米氧化锌 细菌群落 高通量测序 

分 类 号：X172[环境科学与工程—环境科学]