中性粒细胞和巨噬细胞在嗜酸性胃肠炎小鼠各组织中的浸润情况分析  

Analysis of infiltration of neutrophils and macrophages in tissues of eosinophilic gastroenteritis mice

作　　者：徐龙薇 王亚丹[1] 周敏思 吴静[2] Xu Longwei;Wang Yadan;Zhou Minsi;Wu Jing(Department of Gastroenterology,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China;Department of Gastroenterology,Beijing Friendship Hospital,Capital Medical University,National Clinical Research Center for Digestive Diseases,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京世纪坛医院消化内科,北京100038 [2]首都医科大学附属北京友谊医院消化内科国家消化系统疾病临床医学研究中心,北京100050

出　　处：《首都医科大学学报》2025年第2期306-313,共8页Journal of Capital Medical University

基　　金：国家重点研发计划项目(2022YFC3602104);2022年首都卫生发展科研专项(首发2022-2-2025);2021北京市科委首都特色:北京市科技计划课题实施方案(专项B本)(项目编号:Z211100002921028)。

摘　　要：目的研究中性粒细胞和巨噬细胞在嗜酸性胃肠炎小鼠各组织中的浸润情况。方法选取6~8周龄雌性C57BL/6小鼠30只,应用腹腔注射鸡卵清蛋白(ovalbumin,OVA)致敏和灌胃给予OVA的方式诱导野生型小鼠建立嗜酸性胃肠炎模型。通过测量小鼠体质量变化、应用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测小鼠外周血OVA特异性免疫球蛋白E(immunoglobulin E,IgE)和免疫球蛋白G(immunoglobulin G,IgG)水平、应用流式细胞术检测小鼠肠道嗜酸性粒细胞计数等方法验证小鼠嗜酸性胃肠炎是否造模成功。通过流式细胞术检测小鼠外周血中性粒细胞、单核细胞比例,检测脾脏、肠系膜淋巴结、小肠和结肠组织中的中性粒细胞和巨噬细胞比例。结果OVA腹腔致敏和灌胃激发可成功诱导小鼠嗜酸性胃肠炎模型。嗜酸性胃肠炎小鼠外周血单核细胞及脾脏、肠系膜淋巴结和小肠、结肠组织中的巨噬细胞浸润增多,中性粒细胞在嗜酸性胃肠炎小鼠的外周血及小肠、结肠组织中浸润比例增加。结论巨噬细胞在酸性胃肠炎小鼠外周血、肠道引流淋巴结及肠组织中浸润增多,中性粒细胞在嗜酸性胃肠炎小鼠外周血和肠组织中浸润也增多。Objective To study the infiltration of neutrophils and macrophages in the tissues of eosinophilic gastroenteritis mice.Methods Thirty female C57BL/6 mice aged 6-8 weeks were selected to induce the model of eosinophilic gastroenteritis in wild-type mice.They were sensitized by intraperitoneal injection of ovalbumin(OVA)protein and they were gastric stimulated with OVA.The successful modeling of eosinophilic gastroenteritis was verified by measuring the weight change of mice,detecting OVA specific immunoglobulin E(IgE)and Immunoglobulin G(IgG)levels in peripheral blood of mice by enzyme-linked immunosorbent assay(ELISA),and detecting intestinal eosinophils count by flow cytometry.The proportion of neutrophils and monocytes in peripheral blood and the proportion of neutrophils and macrophages in spleen,mesenteric lymph nodes,small intestine and colon tissues were detected by flow cytometry.Results The mice model of eosinophilic gastroenteritis was successfully induced by OVA abdominal sensitization and gavage stimulation.In mice with eosinophilic gastroenteritis,the infiltration of monocytes in peripheral blood,macrophages infiltration of spleen,mesenteric lymph nodes,small intestine and colon tissues were increased.And the neutrophil were increased infiltrated in peripheral blood,small intestine and colon tissues.Conclusion Macrophage and neutrophil were increased infiltrated in peripheral blood,intestinal tissue of mice with eosinophilic gastroenteritis.Furthermore,the infiltration of macrophage was increased in lymphoid tissues,such as spleen and mesenteric lymph nodes of mice with eosinophilic gastroenteritis.

关 键 词：小鼠 嗜酸性胃肠炎 嗜酸性粒细胞 中性粒细胞 巨噬细胞 

分 类 号：R57[医药卫生—消化系统] R392[医药卫生—内科学]