能量限制下补充特定营养素对小鼠运动表现的影响  

Effect of supplementing specific nutrients under calorie restriction on exercise performance in mice

作　　者：袁家奇 王鹏[1] 李韦昉 饶昕 周敏[1] 糜漫天[1] 秦玉[1] YUAN Jiaqi;WANG Peng;LI Weifang;RAO Xin;ZHOU Min;MI Mantian;QIN Yu(Research Center for Nutrition and Food Safety,Chongqing Key Laboratory of Nutrition and Health,Chongqing Medical Nutrition Research Center,Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing,China)

机构地区：[1]陆军军医大学(第三军医大学)军事预防医学系营养与食品安全研究中心,重庆市医学营养研究中心,重庆市营养与健康重点实验室,重庆

出　　处：《陆军军医大学学报》2025年第7期664-673,共10页Journal of Army Medical University

基　　金：后勤科研(2022)。

摘　　要：目的研制一种在能量限制条件下能有效提升运动表现的复合营养制剂,并对其作用效果进行综合评估。方法24只雄性C57BL/6J小鼠(体质量23~26 g,8周龄)随机数字表法分为4组:对照组(CON)、能量限制组(CR)、能量限制+基础营养素组(CRN1)以及能量限制+复合营养素组(CRN2)。所有组进行每周5 d的中等强度跑步训练,每周测量前肢握力,21 d后进行力竭和力竭后运动距离测试以评估运动表现。检测血液生化指标、骨骼肌和肝脏氧化还原生物标志物水平以及组织病理情况。结果干预21 d后,与CON组相比,CR组和CRN1组小鼠跑步力竭距离分别延长278%和289%(P<0.05),血糖、肌肉质量、皮下脂肪和附睾脂肪质量均有所下降(P<0.05);相较于CON组,CRN1组和CRN2组腓肠肌糖原含量显著上升;CRN2组力竭后的运动距离比CON组和CRN1组分别增加52%和36%(P<0.05),前肢握力较其他各组分别提高9%、17%和15%(P<0.05),棕色脂肪质量比CON组和CRN1组显著增加(P<0.05),血糖比CRN1组显著上升(P<0.05),血液低密度脂蛋白胆固醇含量比CON组和CR组都显著减少(P<0.05),腓肠肌谷胱甘肽过氧化物酶较CON组显著提高(P<0.05)。结论能量限制下补充复合营养制剂可以提升小鼠的运动表现,包括促进力竭运动后的疲劳恢复、提高前肢握力。Objective To develop a composite nutritional preparation that can effectively improve exercise performance under calorie restriction(CR)condition.Methods A total of 24 male C57BL/6J mice(weighing 23~26 g,8 weeks old)were randomly divided into control group(CON),CR,CR+basal nutrient group(CRN1),and CR+compound nutrient group(CRN2).All groups underwent moderate-intensity running training 5 d per week,for totally 3 weeks.The grip strength of the forelimbs were measured weekly,and in 3 weeks after training,exhaustion and post-exhaustion distance tests were conducted to evaluate exercise performance.Blood biochemical indicators,levels of skeletal muscle and liver redox biomarkers,and histopathological conditions were measured and observed.Results After 21 d of intervention,the CR group and CRN1 group had the post-exhaustion running distance prolonged by 278%and 289%,respectively,reduced blood glucose level,and decreased muscle mass,subcutaneous fat and epididymal fat mass when compared with the CON group(P<0.05).Compared with the CON group,the CRN1 and CRN2 groups demonstrated significantly higher gastrocnemius glycogen content.The CRN2 group obtained even longer post-exhaustion distance(increased by 52%and 36%respectively,compared with the CON group and CRN1 group,P<0.05),enhanced grip strength of the forelimbs(raised by 9%,17%and 15%,respectively than the CON,CR and CRN1 groups,P<0.05),elevated brown fat mass(compared to the CON group and CRN1 group,P<0.05),increased blood glucose level(compared to the CRN1 group,P<0.05),decreased blood low-density lipoprotein cholesterol level(compared to the CON and CR groups,P<0.05),and increased glutathione peroxidase content in the gastrocnemius muscle(compared to the CON group,P<0.05).Conclusion Supplementing with compound nutritional supplements in mice under CR can promote exercise performance,including improving fatigue recovery after exhaustive exercise and enhancing forelimb grip strength.

关 键 词：能量限制 维生素 矿物质 复合营养素 运动表现 

分 类 号：G804.7[文化科学—运动人体科学] R-332[文化科学—体育学] R151.43[医药卫生]